Owens W E, Watts J L
Hill Farm Research Station, Louisiana State University Agricultural Center, Homer 71040.
J Dairy Sci. 1987 Sep;70(9):1946-51. doi: 10.3168/jds.S0022-0302(87)80236-9.
Addition of milk to Mueller-Hinton susceptibility test medium permitted measurement of milk effect on agar disc diffusion zone diameters obtained from Staphylococcus aureus herd isolates and stock strains. Milk reduced zone diameters for all antimicrobics tested when compared with zones obtained by standard methods. Antimicrobics most affected included novobiocin, streptomycin, gentamycin, tetracycline, and vancomycin. Growth curves of a reference strain of Staphylococcus aureus in Mueller-Hinton broth with and without milk indicated no effect on growth rate of the organism, suggesting the observed effect was due to the action of milk on the antimicrobics being tested. The described method offers a simple in vitro test for milk effects on antimicrobic activity.
在穆勒-欣顿药敏试验培养基中添加牛奶，可测定牛奶对从金黄色葡萄球菌群体分离株和标准菌株获得的琼脂扩散圈直径的影响。与标准方法获得的抑菌圈相比，牛奶使所有测试抗菌药物的抑菌圈直径减小。受影响最大的抗菌药物包括新生霉素、链霉素、庆大霉素、四环素和万古霉素。在含和不含牛奶的穆勒-欣顿肉汤中，金黄色葡萄球菌参考菌株的生长曲线表明牛奶对该菌的生长速率没有影响，这表明观察到的影响是由于牛奶对所测试抗菌药物的作用。所描述的方法为检测牛奶对抗菌活性的影响提供了一种简单的体外试验。
