Department of Analytical Chemistry, Physical Chemistry and Chemical Engineering, University of Alcala, Alcala de Henares E-28871, Madrid, Spain.
Chemical Research Institute "Andrés M. del Río", University of Alcala, Alcala de Henares E-28871, Madrid, Spain.
Lab Chip. 2022 Mar 1;22(5):928-935. doi: 10.1039/d1lc01106e.
Herein, we describe the design of a portable device integrated with micromotors for real-time fluorescence sensing of (bio)markers. The system comprises a universal 3D printed platform to hold a commercial smartphone, which is equipped with an external magnification optical lens (20-400×) and tailor-made emission filters directly attached to the camera, an adjustable sample holder to accommodate a glass slide and laser excitation sources. On a first approach, we illustrate the suitability of the platform using magnetic Janus micromotors modified with fluorescent ZnS@CdSe quantum dots for real-time ON-OFF mercury detection. On a second approach, graphdiyne tubular catalytic micromotors modified with a rhodamine labelled affinity peptide are used for the OFF-ON detection of cholera toxin B. The micromotor-based smartphone for fluorescence sensing approach was compared to a high-performance optical microscope, and similar analytical features were obtained. This versatility allows for easy integration of micromotor fluorescence sensing strategies based on different propulsion mechanisms, allowing for its future use with a myriad of biomarkers and even multiplexed schemes.
在这里，我们描述了一种便携式设备的设计，该设备集成了微马达，用于实时荧光检测（生物）标志物。该系统包括一个通用的 3D 打印平台，用于放置商业智能手机，该智能手机配备了外部放大光学透镜（20-400×）和直接连接到相机的定制发射滤波器，一个可调节的样品架，用于容纳载玻片和激光激发源。在第一种方法中，我们使用带有荧光 ZnS@CdSe 量子点的修饰后的磁性 Janus 微马达说明了该平台的适用性，用于实时 ON-OFF 汞检测。在第二种方法中，使用带有标记了罗丹明的亲和肽的二维碳纳米管管状催化微马达用于霍乱毒素 B 的 OFF-ON 检测。基于微马达的智能手机荧光传感方法与高性能光学显微镜进行了比较，获得了相似的分析特征。这种多功能性允许轻松集成基于不同推进机制的微马达荧光传感策略，为其未来与众多生物标志物甚至多重方案的使用做好了准备。
