Zamze S E, Moxon E R
Department of Paediatrics, John Radcliffe Hospital, Headington, Oxford, UK.
J Gen Microbiol. 1987 Jun;133(6):1443-51. doi: 10.1099/00221287-133-6-1443.
Lipopolysaccharide (LPS) from all six serotype strains of Haemophilus influenzae was similar in composition. The oligosaccharide, of each LPS, was composed of glucose, galactose, heptose and 2-keto-3-deoxyoctonic acid. The lipid A was composed of glucosamine, phosphate and the fatty acids 14:0 and 3-OH 14:0. Each LPS also contained ethanolamine and ethanolamine phosphate, and the oligosaccharides from two strains additionally contained small amounts of glucosamine. Although the LPS was similar in composition, different serotypes had quantitative differences, especially in the galactose content, which correlated with the antigenic specificity of their homologous antisera and with their mobility on SDS-polyacrylamide gel electrophoresis (SDS-PAGE). A survey by SDS-PAGE showed that LPS from strains of the serotypes a, c and d was characteristically of lower Mr than the LPS from most (80%) serotype b strains.
来自流感嗜血杆菌所有六种血清型菌株的脂多糖（LPS）在组成上相似。每种LPS的寡糖由葡萄糖、半乳糖、庚糖和2-酮-3-脱氧辛酸组成。脂质A由葡糖胺、磷酸盐以及脂肪酸14:0和3-羟基-14:0组成。每种LPS还含有乙醇胺和磷酸乙醇胺，并且来自两个菌株的寡糖还额外含有少量葡糖胺。尽管LPS在组成上相似，但不同血清型存在定量差异，尤其是在半乳糖含量方面，这与它们同源抗血清的抗原特异性以及它们在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）上的迁移率相关。通过SDS-PAGE进行调查显示，血清型a、c和d菌株的LPS分子量通常低于大多数（80%）血清型b菌株的LPS。
