College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, China.
Hunan Engineering Laboratory for Pollution Control and Waste, Utilization in Swine Production, Changsha, China.
Int J Phytoremediation. 2022;24(11):1205-1212. doi: 10.1080/15226514.2021.2024134. Epub 2022 Jan 7.
Soil lead (Pb) contamination has caused severe environmental threats and is in urgent need of remediation. This study was aimed to explore the feasibility of using the Miscanthus-microbe combination to reduce Pb pollution in the farmland surrounding a lead-zinc mining area. We have screened three heteroauxin (IAA)-producing microbes ( MR2, TS8, and ZR1) with high Pb tolerance. The IAA-producing ability of the mixed-species was stronger than that of the single bacterium. In pot experiments, the mixed-species of MR2-ZR1 and MR2-TS8 had better performance in enhancing the weight of Miscanthus grass (increased by 22.2-53.6% compared to the control group without inoculating microbes). The remediation efficiency of Pb was significantly higher in the MR2 (30.79%), MR2-TS8 (24.96%), and TS8-ZR1 (21.10%) groups than that in the control group (6.75%). We speculated that MR2 and mixed species of MR2-TS8 and TS8-ZR1 could promote the percentages of activated Pb fractions in soils and increase the Pb uptake of (Lab.). These results implied that the MR2-TS8 mixed-species might be selected as the effective microbial agent to simultaneously enhance the remediation efficiency of Pb-contaminated soils and the biomass of (Lab.).
土壤铅（Pb）污染对环境造成了严重威胁，亟待修复。本研究旨在探讨利用芒草-微生物组合修复矿区周边农田 Pb 污染的可行性。我们筛选了三株具有较高耐铅性的产吲哚乙酸（IAA）微生物（MR2、TS8 和 ZR1）。混合物种的产 IAA 能力强于单一细菌。在盆栽实验中，MR2-ZR1 和 MR2-TS8 混合物种在提高芒草草重方面表现更好（与不接种微生物的对照组相比，增加了 22.2-53.6%）。MR2（30.79%）、MR2-TS8（24.96%）和 TS8-ZR1（21.10%）组的 Pb 修复效率明显高于对照组（6.75%）。我们推测 MR2 和混合物种 MR2-TS8 和 TS8-ZR1 可以促进土壤中活性 Pb 分数的百分比，并增加对芒草的 Pb 吸收。这些结果表明，MR2-TS8 混合物种可能被选为有效微生物剂，同时提高 Pb 污染土壤的修复效率和芒草的生物量。
