Morabito F, Prasthofer E F, Pullen D J, Mahoney D, Downing J R, Crist W M, Grossi C E
University of Alabama at Birmingham.
Leukemia. 1987 Jun;1(6):514-7.
By using monoclonal antibodies specific for T lineage surface antigens, neoplastic T cells from 53 children with T cell acute lymphoblastic leukemia and T cell non-Hodgkin's lymphoma were analyzed and assigned to phenotypically defined stages of T cell maturation. Cells were also analyzed for T cell antigen receptor beta-chain gene and immunoglobulin heavy and light chain gene rearrangements. Clonal rearrangements of T cell antigen receptor beta-chain gene and a germ-line configuration of the immunoglobulin genes were found in cells from all cases. The expression of the terminal deoxynucleotidyl transferase (TdT) in leukemic T cells was studied by both qualitative immunofluorescence and quantitative enzyme immunoassay, and the level of TdT expression was correlated with maturational stages. Lymphoblasts classified as prethymic (3 patients) did not express detectable TdT. In contrast, cells from approximately 60% of the patients with early (15 patients) or intermediate (19 patients) thymocytic phenotypes and approximately 40% of the patients with mature thymocytic phenotype (16 patients) were positive for TdT. Thus, in these leukemic clones TdT was randomly expressed and showed no correlation with maturational stage.
利用针对T谱系表面抗原的单克隆抗体，对53例T细胞急性淋巴细胞白血病和T细胞非霍奇金淋巴瘤患儿的肿瘤性T细胞进行分析，并将其归入T细胞成熟的表型定义阶段。还对细胞进行了T细胞抗原受体β链基因以及免疫球蛋白重链和轻链基因重排的分析。在所有病例的细胞中均发现了T细胞抗原受体β链基因的克隆性重排以及免疫球蛋白基因的种系构型。通过定性免疫荧光和定量酶免疫测定法研究了白血病T细胞中末端脱氧核苷酸转移酶（TdT）的表达情况，并将TdT表达水平与成熟阶段相关联。归类为胸腺前的淋巴母细胞（3例患者）未表达可检测到的TdT。相比之下，约60%早期（15例患者）或中期（19例患者）胸腺细胞表型的患者以及约40%成熟胸腺细胞表型的患者（16例患者）的细胞TdT呈阳性。因此，在这些白血病克隆中，TdT随机表达，与成熟阶段无相关性。
