Cui Dandan, Yang Jin, Lu Bosi, Deng Lansheng, Shen Hong
College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, PR China.
College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, PR China; Guangdong Provincial Key Laboratory of Eco-Circular Agriculture, Guangzhou 510642, PR China.
Int J Biol Macromol. 2022 Feb 15;198:204-213. doi: 10.1016/j.ijbiomac.2021.12.173. Epub 2022 Jan 4.
Mantis shrimp waste (Oratosquilla oratoria) is a good source of chitin. The applicability of microwave-assisted organic acids and proteases for extracting chitin from mantis shrimp shell waste was evaluated, and the extracted-chitin was characterized by scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and thermogravimetric analysis (TGA). Moreover, the effects of nanochitin on the growth of pak choi (Brassica campestris L.ssp.) were also investigated. The results indicated that alkaline protease (4000 U/g, microwave heating at 60 °C, 10 min) and malic acid (5%, 320 W, 5 min) exhibited excellent potential for deproteinizing and demineralizing shells. The deproteinization and demineralization yields were 92.78% and 94.11%, respectively, and the chitin yield was 15.6%. The extracted-chitin had a highly porous structure and exhibited excellent crystallinity and thermostability compared with chitin prepared by traditional chemical methods. Furthermore, 0.003% nanochitin significantly enhanced photosynthesis, which improved the pak choi fresh weight by 22.94%, and improved the accumulation of isothiocyanates in its leaves. This study provides an alternative approach for the high-value utilization of mantis shrimp waste, and reveals the potential of chitin for application in agricultural production.
虾蛄废弃物（口虾蛄）是几丁质的良好来源。评估了微波辅助有机酸和蛋白酶从虾蛄壳废弃物中提取几丁质的适用性，并通过扫描电子显微镜（SEM）、傅里叶变换红外光谱（FTIR）、X射线衍射（XRD）和热重分析（TGA）对提取的几丁质进行了表征。此外，还研究了纳米几丁质对小白菜（Brassica campestris L.ssp.）生长的影响。结果表明，碱性蛋白酶（4000 U/g，60℃微波加热10分钟）和苹果酸（5%，320W，5分钟）在壳脱蛋白和脱矿方面具有优异的潜力。脱蛋白率和脱矿率分别为92.78%和94.11%，几丁质产率为15.6%。与传统化学方法制备的几丁质相比，提取的几丁质具有高度多孔的结构，表现出优异的结晶度和热稳定性。此外，0.003%的纳米几丁质显著增强了光合作用，使小白菜鲜重提高了22.94%，并促进了其叶片中异硫氰酸盐的积累。本研究为虾蛄废弃物的高值利用提供了一种替代方法，并揭示了几丁质在农业生产中的应用潜力。
