Department of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha, Hunan, China.
Shanghai Key Lab of Forensic Medicine, Key Lab of Forensic Science, Ministry of Justice, Shanghai, China.
Forensic Sci Int. 2024 Aug;361:112136. doi: 10.1016/j.forsciint.2024.112136. Epub 2024 Jul 2.
Etomidate as a non-barbiturate sedative, has central inhibitory effect and addiction and has been listed as a controlled drug in some countries due to the abusing trend nowadays. Therefore, rapid and sensitive detection of etomidate is of great significance. In this work, a novel fluorescent sensing probe (CuNCs@MIPs) based on copper nanoclusters (CuNCs) and molecular imprinted polymers (MIPs) has been firstly reported. CuNCs was environment-friendly synthesized using poly(vinylpyrrolidone) as a template and ascorbic acid as a reducing agent. After functionalized with molecular imprinting technique, the CuNCs@MIPs probe has special binding cavities on surface to target etomidate, causing the fluorescence intensity rapidly decrease, which confirmed it has excellent sensitivity, selectivity and stability. Under optimal conditions, the fluorescent sensing probe presented high precision linear relationship for etomidate in range of 10-500 ng/ml with detection limit of 10 ng/ml, and the whole detection process was completed within 10 min. This sensing method has also been applied to real samples detection, still demonstrated excellent feasibility in electronic cigarette liquids and urine. More importantly, compared with previous methods, this fluorescent sensing method has advantages such as rapid, simple and easy to operate. Collectively, the proposed CuNCs@MIPs sensing probe has good fluorescence characteristics and simple synthesis strategy, showed a great potential in etomidate detection and application.
依托咪酯作为一种非巴比妥类镇静剂，具有中枢抑制作用和成瘾性，由于目前滥用趋势，已在一些国家被列为管制药物。因此，快速、灵敏地检测依托咪酯具有重要意义。在本工作中，首次报道了一种基于铜纳米簇（CuNCs）和分子印迹聚合物（MIPs）的新型荧光传感探针（CuNCs@MIPs）。采用环境友好的方法，以聚（乙烯基吡咯烷酮）为模板、抗坏血酸为还原剂合成了 CuNCs。经分子印迹技术功能化后，CuNCs@MIPs 探针表面具有针对依托咪酯的特殊结合腔，导致荧光强度迅速下降，证实其具有优异的灵敏度、选择性和稳定性。在最佳条件下，荧光传感探针对依托咪酯在 10-500ng/ml 范围内呈现出高精密线性关系，检测限为 10ng/ml，整个检测过程在 10min 内完成。该传感方法还应用于实际样品检测，在电子烟液和尿液中仍表现出优异的可行性。更重要的是，与以前的方法相比，这种荧光传感方法具有快速、简单、易于操作等优点。综上所述，所提出的 CuNCs@MIPs 传感探针具有良好的荧光特性和简单的合成策略，在依托咪酯检测和应用方面具有很大的潜力。
