Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation and Key Laboratory of Central South Fast-Growing Timber Cultivation of Forestry Ministry of China, Guangxi Forestry Research Institute, Nanning, 530002, People's Republic of China.
College of Landscape Architecture and Forestry, Qingdao Agricultural University, Qingdao, 266109, People's Republic of China.
Sci Rep. 2022 Jan 7;12(1):258. doi: 10.1038/s41598-021-04406-1.
The radial change (RC) of tree stem is the process of heartwood formation involved in complex molecular mechanism. Chinese fir (Cunninghamia lanceolata (Lamb.) Hook.), an evergreen species, is an important fast-growing timber tree in southern China. In this study, the top four stable genes (IDH, UBC2, RCA and H2B) were selected in RC tissues of 15 years old Chinese fir stem (RC15) and the genes (H2B, 18S, TIP41 and GAPDH) were selected in RC tissues of 30 years old Chinese fir stem (RC30). The stability of the reference genes is higher in RC30 than in RC15. Sixty-one MYB transcripts were obtained on the PacBio Sequel platform from woody tissues of one 30 years old Chinese fir stem. Based on the number of MYB DNA-binding domain and phylogenetic relationships, the ClMYB transcripts contained 21 transcripts of MYB-related proteins (1R-MYB), 39 transcripts of R2R3-MYB proteins (2R-MYB), one transcript of R1R2R3-MYB protein (3R-MYB) belonged to 18 function-annotated clades and two function-unknown clades. In RC woody tissues of 30 years old Chinese fir stem, ClMYB22 was the transcript with the greatest fold change detected by both RNA-seq and qRT-PCR. Reference genes selected in this study will be helpful for further verification of transcript abundance patterns during the heartwood formation of Chinese fir.
树干的径向变化（RC）是心材形成所涉及的复杂分子机制的过程。杉木（Cunninghamia lanceolata（Lamb.）Hook.）是常绿树种，是中国南方重要的速生用材树种。本研究在 15 年生杉木 RC 组织中选择了前 4 个稳定基因（IDH、UBC2、RCA 和 H2B），在 30 年生杉木 RC 组织中选择了 4 个稳定基因（H2B、18S、TIP41 和 GAPDH）。在 RC30 中，参考基因的稳定性高于 RC15。从一棵 30 年生的杉木的木质部组织中，在 PacBio Sequel 平台上获得了 61 个 MYB 转录本。基于 MYB DNA 结合域的数量和系统发育关系，ClMYB 转录本包含 21 个 MYB 相关蛋白（1R-MYB）转录本、39 个 R2R3-MYB 蛋白转录本（2R-MYB）、1 个 R1R2R3-MYB 蛋白转录本（3R-MYB），分为 18 个功能注释的分支和 2 个功能未知的分支。在 30 年生杉木 RC 木质部组织中，ClMYB22 是 RNA-seq 和 qRT-PCR 检测到的转录本变化倍数最大的转录本。本研究中选择的参考基因将有助于进一步验证杉木心材形成过程中转录本丰度模式。
