Industrial Technology Innovation Center of Ibaraki Prefecture, Nagaoka, Japan.
Meguro Institute Co. Ltd., Osaka, Japan.
J Food Biochem. 2022 Feb;46(2):e14046. doi: 10.1111/jfbc.14046. Epub 2022 Jan 7.
Bacillus subtilis BN strain (BN strain) was isolated from natto, a traditional Japanese fermented soybean food product. The present study investigated the Th1 responses of the BN strain on a mouse macrophage cell line, J774.1. In cell cultures, the BN strain (spore cell cultured in Schaeffer's sporulation media) significantly increased the production of interleukin (IL-)12 protein. The BN strain induced the mRNA expression of M1 polarization genes, such as inducible nitric oxide synthase and IL-12p40 mRNA, and suppressed the mRNA expression of intracellular marker genes of M2 polarization, such as arginase 1 mRNA. The BN strain downregulated the mRNA expression of Toll-like receptor 4 (TLR4), while it upregulated the mRNA expression of TLR2, MyD88, and nuclear factor kappa B (NF-κB). The production of IL-12 protein induced by the BN strain was decreased by inhibitors of MyD88, NF-κB, and IκB kinase. Moreover, the production of IL-12 was strongly suppressed by neutralizing antibody against TLR2. These results suggest that the BN strain promotes Th1 response via TLR2 signal in mouse M1 macrophage. PRACTICAL APPLICATIONS: Bacillus subtilis is known to have beneficial effects for the host. B. subtilis BN stain (BN strain) was isolated from natto, a traditional Japanese fermented soybean food product. The effects of the BN strain on the Th1 response in macrophage cell cultures were investigated in this work. We found that the spore cells of BN strain promoted the production of Th1-type cytokine, and induced macrophage M1 polarization via Toll-like receptor 2. This study can serve as a significant reference for the development of functional food and feed with immunostimulatory effects. Over time, new food and feed products containing the BN strain may emerge, such as Juice, powder, and tablet.
枯草芽孢杆菌 BN 株（BN 株）是从豆豉，一种传统的日本发酵大豆食品中分离出来的。本研究探讨了 BN 株在小鼠巨噬细胞系 J774.1 上的 Th1 反应。在细胞培养中，BN 株（在 Scha effer 的孢子形成培养基中培养的孢子细胞）显著增加了白细胞介素（IL-）12 蛋白的产生。BN 株诱导 M1 极化基因的 mRNA 表达，如诱导型一氧化氮合酶和 IL-12p40mRNA，并抑制 M2 极化的细胞内标记基因的 mRNA 表达，如精氨酸酶 1mRNA。BN 株下调 Toll 样受体 4（TLR4）的 mRNA 表达，同时上调 TLR2、MyD88 和核因子 kappa B（NF-κB）的 mRNA 表达。BN 株诱导的 IL-12 蛋白的产生被 MyD88、NF-κB 和 IκB 激酶抑制剂所减少。此外，TLR2 的中和抗体强烈抑制了 IL-12 的产生。这些结果表明，BN 株通过小鼠 M1 巨噬细胞中的 TLR2 信号促进 Th1 反应。实际应用：枯草芽孢杆菌对宿主有有益的影响。枯草芽孢杆菌 BN 株（BN 株）是从豆豉，一种传统的日本发酵大豆食品中分离出来的。本工作研究了 BN 株对巨噬细胞培养中 Th1 反应的影响。我们发现，BN 株的孢子细胞通过 Toll 样受体 2 促进 Th1 型细胞因子的产生，并诱导巨噬细胞 M1 极化。这项研究可以为具有免疫刺激作用的功能性食品和饲料的开发提供重要参考。随着时间的推移，可能会出现含有 BN 株的新的食品和饲料产品，如 Juice、powder 和 tablet。
