Fujii Keisuke, Kubo Yuji, Noguchi Tomotsugu, Tobita Keisuke
Industrial Technology Innovation Center of Ibaraki Prefecture, Nagaoka 311-3195, Ibaraki, Japan.
Foods. 2023 Jan 9;12(2):313. doi: 10.3390/foods12020313.
natto is used in the production of natto, a traditional fermented soy food, and has beneficial immunomodulatory effects in humans. Single-stranded RNA (ssRNA) viruses, including influenza and coronavirus, often cause global pandemics. We proposed a human cell culture model mimicking ssRNA viral infection and investigated the ability of natto to induce antiviral effects in the model. The gene expressions were analyzed using quantitative real-time reverse transcription PCR. M1-phenotype macrophages derived from THP-1 cells strongly express the Toll-like receptor 8 (76.2-hold), CD80 (64.2-hold), and CCR7 (45.7-hold) mRNA compared to M0 macrophages. One µg/mL of resiquimod (RSQ)-stimulation induced the expression of IRF3 (1.9-hold), CXCL10 (14.5-hold), IFNβ1 (3.5-hold), ISG20 (4.4-hold), and MxA (1.7-hold) mRNA in the M1-phenotype macrophages. Based on these results, the RSQ-stimulated M1-phenotype macrophages were used as a cell culture model mimicking ssRNA viral infection. Moreover, the natto XF36 strain induced the expression of genes associated with antiviral activities (IFNβ1, IFNλ1, ISG20, and RNase L) and anti-inflammatory activities (IL-10) in the cell culture model. Thus, it is suggested that the XF36 suppresses viral infections and excessive inflammation by inducing the expression of genes involved in antiviral and anti-inflammatory activities.
纳豆激酶用于制作纳豆，一种传统的发酵豆制品，对人体具有有益的免疫调节作用。包括流感病毒和冠状病毒在内的单链RNA（ssRNA）病毒常常引发全球大流行。我们提出了一种模拟ssRNA病毒感染的人类细胞培养模型，并研究了纳豆在该模型中诱导抗病毒效应的能力。使用定量实时逆转录PCR分析基因表达。与M0巨噬细胞相比，源自THP-1细胞的M1表型巨噬细胞强烈表达Toll样受体8（76.2倍）、CD80（64.2倍）和CCR7（45.7倍）mRNA。1μg/mL瑞喹莫德（RSQ）刺激可诱导M1表型巨噬细胞中IRF3（1.9倍）、CXCL10（14.5倍）、IFNβ1（3.5倍）、ISG20（4.4倍）和MxA（1.7倍）mRNA的表达。基于这些结果，RSQ刺激的M1表型巨噬细胞被用作模拟ssRNA病毒感染的细胞培养模型。此外，纳豆XF36菌株在细胞培养模型中诱导了与抗病毒活性（IFNβ1、IFNλ1、ISG20和RNase L）和抗炎活性（IL-10）相关的基因表达。因此，提示XF36通过诱导参与抗病毒和抗炎活性的基因表达来抑制病毒感染和过度炎症。
