BACKGROUND AND OBJECTIVES

Colorectal cancer (CRC) is a heterogeneous disease of the colon or rectum arising from adenoma precursors and serrated polyps. Recently, probiotics have been proposed as an effective and potential therapeutic approach for CRC prevention and treatment. Probiotics have been shown to alleviate inflammation by restoring the integrity of the mucosal barrier and impeding cancer progression.

MATERIALS AND METHODS

In this study, we aimed to investigate the immunomodulatory effects of live and UV-killed natto on the inflammatory response in CRC. Caco-2 cells were exposed to various concentrations of live and UV- killed natto, and cell viability was assessed using MTT assay. Gene expression analysis of IL-10, TGF-β, TLR2 and TLR4 was performed using RT-qPCR.

RESULTS

Our findings showed that both live and UV-killed natto caused significant reduction in inflammatory response by decreasing the gene expression of TLR2 and TLR4, and enhancing the gene expression of IL-10 and TGF-β in Caco-2 cells as compared to control group.

CONCLUSION

The results of this study suggest that live and UV-killed natto may hold potential as a therapeutic supplement for modulating inflammation in CRC.