Luo Shan, Jing Jiaxing, Zhang Ying, Yu Wentao, Gao Weijuan
The Graduate College, Hebei University of Chinese Medicine, 050091 Shijiazhuang, Hebei, China.
The Basic Medicine College, Hebei University of Chinese Medicine, 050200 Shijiazhuang, Hebei, China.
J Integr Neurosci. 2021 Dec 30;20(4):847-859. doi: 10.31083/j.jin2004087.
We used network pharmacology to predict the correlation between the pathway of Bushenhuoxue formula in the treatment of vascular dementia and carried out experiments to verify the correlation between drug composition and disease. By screening the active components and key targets through various databases and drawing the topological network diagram, we obtained 502 effective compound targets, 601 disease targets, 95 disease-related compound targets, and 162 pathways. The pathway related to vascular dementia may be neurodegeneration-multiple diseases, PI3K-Akt signaling pathway, Mitogen-activated protein kinase signaling pathway, or HIF-1 signaling pathway. By detecting the learning and memory ability of vascular dementia rats, the morphology of the hippocampus under the electron microscope, the degree of neuronal damage, and autophagy-related proteins, the results showed that the Bushenhuoxue formula could improve the neuronal injury induced by ischemia in the hippocampus, down-regulate the level of autophagy, and thereby improve learning and memory. Therefore, the Bushenhuoxue formula may improve the ischemic injury of neurons by regulating the mechanism of neuronal autophagy.
我们运用网络药理学预测补肾活血方治疗血管性痴呆的通路相关性，并开展实验验证药物成分与疾病之间的相关性。通过各种数据库筛选活性成分和关键靶点并绘制拓扑网络图，我们获得了502个有效化合物靶点、601个疾病靶点、95个疾病相关化合物靶点以及162条通路。与血管性痴呆相关的通路可能是神经退行性变-多种疾病、PI3K-Akt信号通路、丝裂原活化蛋白激酶信号通路或HIF-1信号通路。通过检测血管性痴呆大鼠的学习记忆能力、电镜下海马形态、神经元损伤程度以及自噬相关蛋白，结果表明补肾活血方能够改善海马缺血诱导的神经元损伤，下调自噬水平，从而改善学习记忆。因此，补肾活血方可能通过调节神经元自噬机制改善神经元缺血损伤。
