Brooks B A, Eidelberg E, Morgan W W
Department of Physiology, University of Texas Health Science Center, San Antonio 78284-7756.
Brain Res. 1987 Sep 1;419(1-2):329-32. doi: 10.1016/0006-8993(87)90602-0.
Monkeys were required to press a lever rapidly for food, using either the right or the left hand. After stable baseline performance was established, MPTP (N-methyl-4-phenyl-2,3,5,6-tetrahydropyridine) was injected into the internal carotid of one side via a transfemoral catheter. The onset and time course of clinically severe, Parkinson-like symptoms were paralleled by a significant decrease of bar-pressing activity in the side contralateral to injection, while the forelimb in the unaffected side continued normal pressing. The unilaterality of effects was confirmed biochemically after sacrifice by a 95% drop in striatal dopamine (DA) levels of the injected compared to the uninjected hemisphere. The results show that hemiparkinsonism achieved by carotid injection is very stable; that normal motor behavior is maintained on the unaffected side and that goal-achieving strategies remain active, but that the affected side is unable to execute the task unless extrinsic levodopa is provided.
要求猴子用右手或左手快速按压杠杆以获取食物。在建立稳定的基线表现后，通过经股动脉导管将MPTP（N-甲基-4-苯基-2,3,5,6-四氢吡啶）注入一侧颈内动脉。临床上严重的帕金森样症状的发作和时间进程与注射对侧的压杆活动显著减少平行，而未受影响侧的前肢继续正常按压。在牺牲动物后通过生化方法证实了效应的单侧性，与未注射半球相比，注射侧纹状体多巴胺（DA）水平下降了95%。结果表明，通过颈动脉注射实现的偏侧帕金森病非常稳定；未受影响侧维持正常运动行为，实现目标的策略仍然活跃，但除非提供外源性左旋多巴，否则受影响侧无法执行任务。
