体外产生对结肠癌细胞的肿瘤特异性淋巴细胞反应。与正常结肠黏膜细胞的比较。
In vitro generation of tumour-specific lymphocyte reactivity to colonic carcinoma cells. Comparison with normal colonic mucosa cells.
作者信息
Kuppner M, Wilkinson S, Casson E, Eremin O
机构信息
Department of Surgery, University of Aberdeen, Scotland.
出版信息
Cancer Immunol Immunother. 1987;25(3):209-14. doi: 10.1007/BF00199149.
Purified tumour cells and normal mucosa cells from fresh human colorectal cancer resection specimens, and T-cell-enriched autologous peripheral blood lymphocytes, were mixed in short-term (6 day) mixed lymphocyte-tumour cell (MLTC) microcultures. Lymphocyte stimulation was measured by 3H-thymidine uptake, and a stimulation index (SI = [lymphocytes vs tumour cells (cpm)-tumour cells (cpm)]/[lymphocytes (cpm)] greater than 3 was regarded as significant. Significant lymphocyte reactivity was found in 10/15 patients with colon carcinoma. However, 1 patient with autologous tumour reactivity, also showed significant stimulation against autologous normal mucosa cells, suggesting tumour-associated reactivity. Maximum stimulation occurred most frequently at a lymphocyte:tumour cell ratio of 2:1 and with nylon wool-passaged lymphocytes.
从新鲜的人类结直肠癌切除标本中获取的纯化肿瘤细胞和正常黏膜细胞，以及富含T细胞的自体外周血淋巴细胞，在短期（6天）混合淋巴细胞-肿瘤细胞（MLTC）微培养中混合。通过³H-胸腺嘧啶核苷摄取来测量淋巴细胞刺激，刺激指数（SI = [淋巴细胞与肿瘤细胞（每分钟计数）-肿瘤细胞（每分钟计数）]/[淋巴细胞（每分钟计数）]大于3被视为显著。在15例结肠癌患者中有10例发现显著的淋巴细胞反应性。然而，1例具有自体肿瘤反应性的患者，对自体正常黏膜细胞也表现出显著刺激，提示存在肿瘤相关反应性。最大刺激最常出现在淋巴细胞与肿瘤细胞比例为2:1且使用经尼龙毛柱处理的淋巴细胞时。
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