Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Zhejiang Provincial Clinical Research Center for Oral Disease, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Cancer Center of Zhejiang University, Hangzhou, 310006, People's Republic of China.
Department of Stomatology, Zhejiang University Hospital, Zhejiang University, Hangzhou, People's Republic of China.
Clin Oral Investig. 2022 Mar;26(3):3213-3225. doi: 10.1007/s00784-021-04303-x. Epub 2022 Jan 8.
We investigated the inhibitory effects of captopril on matrix metalloproteinases (MMPs) and its effect as a primer on dentin bonding durability.
One hundred fifty human third molars were selected. Flat surfaces of the middle dentin were exposed, etched 15 s, and followed by pretreatment with a primer for 60 s, including distilled water (control, the negative control primer), 2% chlorhexidine digluconate (CHD, the positive control primer), and captopril solution. Inhibitory effects of primers on MMPs were evaluated by hydroxyproline and gelatinase activity tests. All primers were applied on dentin followed by bonding. Some of the samples were sliced into slabs, placed in a fluorescent solution containing gelatin, and incubated for in situ zymography. Some were cut into sticks, and after aging for 1 day, 12 months, or 24 months, microtensile bonding strength was tested. Some were cut into slabs, aged for 1 day, 12 months, or 24 months, and taken out for nanoleakage tests to reveal interface defects.
Hydroxyproline and gelatinase activity analyses showed that captopril exerted better inhibitory effects on MMPs, relative to 2% CHD (p < 0.05). A 0.2% captopril aqueous solution (0.2% CapW) was chosen to apply to the dentin. In situ zymography showed that inhibitory effects of captopril on gelatinase were significantly higher compared to 2% CHD (p < 0.01). Microtensile strength revealed that the bonding effects of the 0.2% CapW group lasted longer, compared to the control and 2% CHD groups (p < 0.05). Interface defects, detected by nanoleakage, were significantly reduced in the 0.2% CapW group, compared to the control and 2% CHD groups (p < 0.05).
Captopril inhibits dentin MMP activities and effectively improves dentin bonding durability.
Captopril is a promising dentin bonding primer for improving bonding durability.
我们研究了卡托普利对基质金属蛋白酶（MMPs）的抑制作用及其作为底漆对牙本质粘结耐久性的影响。
选择 150 个人类第三磨牙。暴露中间牙本质的平面，蚀刻 15 秒，然后用底漆预处理 60 秒，包括蒸馏水（阴性对照底漆）、2%葡萄糖酸氯己定（阳性对照底漆）和卡托普利溶液。通过羟脯氨酸和明胶酶活性试验评估底漆对 MMPs 的抑制作用。所有底漆均应用于牙本质，然后进行粘结。一些样本被切成薄片，放入含有明胶的荧光溶液中，进行原位酶谱分析。一些被切成小棒，经过 1 天、12 个月或 24 个月老化后，进行微拉伸粘结强度测试。一些被切成薄片，经过 1 天、12 个月或 24 个月老化后，进行纳米渗漏测试以揭示界面缺陷。
羟脯氨酸和明胶酶活性分析表明，卡托普利对 MMPs 的抑制作用优于 2%葡萄糖酸氯己定（p<0.05）。选择 0.2%卡托普利水溶液（0.2%CapW）应用于牙本质。原位酶谱分析表明，卡托普利对明胶酶的抑制作用明显高于 2%葡萄糖酸氯己定（p<0.01）。微拉伸强度显示，与对照组和 2%葡萄糖酸氯己定组相比，0.2%CapW 组的粘结效果持续时间更长（p<0.05）。纳米渗漏检测到的界面缺陷在 0.2%CapW 组明显减少，与对照组和 2%葡萄糖酸氯己定组相比（p<0.05）。
卡托普利抑制牙本质 MMP 活性，有效提高牙本质粘结耐久性。
卡托普利是一种有前途的牙本质粘结底漆，可提高粘结耐久性。
