Shailendra Mashalkar, Bhandari Sarita, Kulkarni Sangeeta, Janavathi K, Ghatole Kiran
Department of Conservative Dentistry and Endodontics, Al Badar Dental College and Hospital, Gulbarga, Karnataka, India.
J Conserv Dent. 2019 Nov-Dec;22(6):598-601. doi: 10.4103/JCD.JCD_236_19. Epub 2020 Aug 20.
The objective was to determine a new experimental material, indomethacin's inhibitory effect on the enzymatic activity of dentin collagen.
Fifteen freshly extracted teeth were collected and stored at 4°C until use. Enamel, roots, and remnant pulp tissue were removed, and dentin powder was obtained by pulverizing liquid nitrogen-frozen coronal dentin with a mortar pestle. The obtained protein extract from human dentin powder was treated with indomethacin and incubated. The inhibition of enzymatic activity was analyzed using plate assay method and zymographic analysis.
Plate assay method and zymograms showed that indomethacin-treated samples inhibited dentin enzymatic activity.
Bond strength at the dentin adhesive interface decreases because of the hydrolytic degradation of dentin collagen. The inhibition of enzymes responsible for collagen degradation may improve the bond strength durability. This study demonstrates the efficacy of indomethacin in inhibiting enzymatic activity.
本研究旨在确定一种新的实验材料——吲哚美辛对牙本质胶原蛋白酶活性的抑制作用。
收集15颗新鲜拔除的牙齿，保存在4°C环境中备用。去除牙釉质、牙根和残留牙髓组织，用研钵杵将液氮冷冻的冠部牙本质研磨成牙本质粉末。从人牙本质粉末中提取的蛋白质提取物用吲哚美辛处理并孵育。采用平板分析法和酶谱分析法分析酶活性的抑制情况。
平板分析法和酶谱分析表明，经吲哚美辛处理的样品抑制了牙本质酶活性。
由于牙本质胶原蛋白的水解降解，牙本质粘结界面的粘结强度降低。抑制负责胶原蛋白降解的酶可能会提高粘结强度的耐久性。本研究证明了吲哚美辛在抑制酶活性方面的有效性。
