Bai Yunpeng, Chen Qingliang, Zhu Xiaolong, Jiang Nan, Li Ximing, Guo Zhigang
Chest Hospital, Tianjin University, Tianjin 300222, China.
Tianjin Chest Hospital, Tianjin Medical University, Tianjin 300222, China.
Evid Based Complement Alternat Med. 2021 Dec 31;2021:6251559. doi: 10.1155/2021/6251559. eCollection 2021.
The patency and quality of transplanted great saphenous vein (GSV) can seriously influence the physical state and life quality of patients who accepted the coronary artery bypass grafting (CABG). Quercetin is known for antioxidant, antithrombotic, anti-inflammatory, and antitumor properties. In this study, we examined the protection of quercetin to the great saphenous vein from oxidative and inflammatory damage.
The GSVs were collected from 15 patients undergoing CABG and cultured. Treated the veins by HO and detected the NO, SOD, and MDA content by the relevant kits to explore the quercetin protection against oxidative damage. Then, for another group of GSVs, sheared them and detected the inflammatory cytokines, such as IL-6, TNF, CCL20, PCNA, and VEGF. Collect the veins for H&E staining and PCNA and VEGF immunofluorescent staining.
Pretreatment by quercetin reduced the production of NO and MDA induced by HO, and increased SOD activity. Quercetin also supressed the mRNA expressions of IL-6, TNF after mechanical damage and had no influence on CCL20 and VEGF. Consistent with the lower expression of PCNA treated by quercetin, the vein intima was thinner.
These results demonstrated that quercetin protects GSVs by reducing the oxidative damage and inflammatory response and also suppresses the abnormal thickening of venous endothelium by inhibiting cell proliferation. It reminded that, to some extent, quercetin has the potential to release the great saphenous vein graft damage.
移植大隐静脉（GSV）的通畅性和质量会严重影响接受冠状动脉旁路移植术（CABG）患者的身体状况和生活质量。槲皮素以其抗氧化、抗血栓形成、抗炎和抗肿瘤特性而闻名。在本研究中，我们研究了槲皮素对大隐静脉氧化和炎症损伤的保护作用。
从15例行CABG的患者中采集大隐静脉并进行培养。用HO处理静脉，并用相关试剂盒检测NO、SOD和MDA含量，以探讨槲皮素对氧化损伤的保护作用。然后，对另一组大隐静脉进行剪切，检测IL-6、TNF、CCL20、PCNA和VEGF等炎性细胞因子。收集静脉进行苏木精-伊红（H&E）染色以及PCNA和VEGF免疫荧光染色。
槲皮素预处理可降低HO诱导的NO和MDA生成，并增加SOD活性。槲皮素还可抑制机械损伤后IL-6、TNF的mRNA表达，且对CCL20和VEGF无影响。与槲皮素处理后PCNA表达降低一致，静脉内膜变薄。
这些结果表明，槲皮素通过减少氧化损伤和炎症反应来保护大隐静脉，还通过抑制细胞增殖来抑制静脉内皮异常增厚。这提示，在一定程度上，槲皮素具有减轻大隐静脉移植损伤的潜力。
