Mamat Noorhidayah, Abu Arpah, Yusoff Norma-Rashid
Institute of Biological Science, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia. E-mail:
Zool Stud. 2021 Aug 26;60:e47. doi: 10.6620/ZS.2021.60-47. eCollection 2021.
Studies on Odonata have gained attention worldwide as well as locally in Malaysia. Although there is a wealth of data available to be utilized for solving taxonomic problems, ecological and behavioural research areas are more favoured than taxonomy and systematics. Thus, there are confusions over how to correctly identify closely related and sympatric species, especially in female odonates. One such example is in the genus . Consequently, the present study focuses on taxonomic work, employing multi-approaches in the form of morphological (morphological diagnostics, Field Emission Scanning Electron Microscope (FESEM) and geometric morphometric analysis), applying the molecular technique. Seventeen morphological characteristics were created to differentiate between the females of spp. A FESEM was used on the female's ovipositor to focus on the anal appendages and sheathing valve (V3). Also, the phylogenetic patterns expressed by and 16S rRNA genes, and canonical variate analysis for the wing geometric morphometric revealed three clusters that supported the distinction of the group. In summary, this study effectively developed an integrated approach of classic morphological and trendy molecular, combined with FESEM microscopy techniques, which provided corroborative evidence and resolved taxonomic uncertainties.
对蜻蜓目昆虫的研究在全球以及马来西亚本地都受到了关注。尽管有大量数据可用于解决分类学问题,但生态和行为研究领域比分类学和系统学更受青睐。因此,对于如何正确识别亲缘关系密切且同域分布的物种存在困惑,尤其是在雌性蜻蜓目昆虫中。一个这样的例子就是 属。因此,本研究聚焦于分类学工作,采用形态学(形态诊断、场发射扫描电子显微镜(FESEM)和几何形态计量分析)等多种方法,并应用分子技术。创建了17个形态特征来区分 属物种的雌性个体。使用FESEM对雌性昆虫的产卵器进行观察,重点关注肛附器和鞘瓣(V3)。此外,由 和16S rRNA基因所表达的系统发育模式,以及翅几何形态计量的典型变量分析揭示了三个聚类,支持了 类群的区分。总之,本研究有效地开发了一种经典形态学与前沿分子技术相结合的综合方法,并结合FESEM显微镜技术,提供了确凿证据并解决了分类学上的不确定性。