Department of Preventive Medicine, Feinberg School of Medicine, Northwestern University, Evanston, IL, United States.
Department of Research and Evaluation, Kaiser Permanente Southern California, Pasadena, CA, United States.
Front Public Health. 2021 Dec 23;9:730369. doi: 10.3389/fpubh.2021.730369. eCollection 2021.
Increasing evidence suggests that exposure to air pollution during pregnancy is associated with adverse pregnancy outcomes. However, biomarkers associated with air pollution exposure are widely lacking and often transient. In addition, ascertaining biospecimens during pregnacy to assess the prenatal environment remains largely infeasible. To address these challenges, we investigated relationships between air pollution exposure during pregnancy and human serum albumin Cys (HSA-Cys) adducts in newborn dried blood spots (DBS) samples, which captures an integration of perinatal exposures to small reactive molecules in circulating blood. Newborn DBS were obtained from a state archive for a cohort of 120 children born at one Kaiser Permanente Southern California (KPSC) hospitals in 2007. These children were selected to maximize the range of residential air pollution exposure during the entire pregnancy to PM, PM, NO, O, based on monthly estimates interpolated from regulatory monitoring sites. HSA-Cys adducts were selected based on previously reported relationships with air pollution exposure and oxidative stress. Six adducts measured in newborn DBS samples were associated with air pollution exposures during pregnancy; these included direct oxidation products, adducts formed with small thiol compounds, and adducts formed with reactive aldehydes. Two general trends were identified: Exposure to air pollution late in pregnancy (i.e., in the last 30 days) was associated with increased oxidative stress, and exposure to air pollution earlier in pregnancy (i.e., not in the last 30 days) was associated with decreased oxidative stress around the time of birth. Air pollution exposure occurring during pregnancy can alter biology and leave measurable impacts on the developing infant captured in the newborn DBS adductome, which represents a promising tool for investigating adverse birth outcomes in population-based studies.
越来越多的证据表明，孕妇暴露于空气污染中与不良妊娠结局有关。然而，与空气污染暴露相关的生物标志物广泛缺乏，且往往是短暂的。此外，在怀孕期间确定生物样本以评估产前环境在很大程度上仍然不可行。为了解决这些挑战，我们研究了孕妇暴露于空气污染与新生儿干血斑（DBS）样本中人血清白蛋白半胱氨酸（HSA-Cys）加合物之间的关系，该样本捕捉了循环血液中小反应性分子的围产期暴露情况。从加利福尼亚州南部 Kaiser Permanente （KPSC）的一家医院的一个队列中获得了新生儿 DBS，该队列中有 120 名儿童于 2007 年出生。这些儿童是根据从监管监测站点内插的每月估计值，为最大限度地扩大整个怀孕期间对 PM、PM、NO、O 的住宅空气污染暴露范围而选择的。HSA-Cys 加合物是根据先前报道的与空气污染暴露和氧化应激的关系选择的。在新生儿 DBS 样本中测量的六种加合物与怀孕期间的空气污染暴露有关；这些包括直接氧化产物、与小硫醇化合物形成的加合物以及与反应性醛形成的加合物。确定了两个总体趋势：妊娠晚期（即最后 30 天）暴露于空气污染与氧化应激增加有关，而妊娠早期（即最后 30 天内）暴露于空气污染与出生时氧化应激降低有关。怀孕期间的空气污染暴露会改变生物学，并在新生儿 DBS 加合物组中留下可测量的对发育中婴儿的影响，这代表了在基于人群的研究中调查不良出生结局的有前途的工具。
