Sternberger N H, Sternberger L A
Department of Anatomy, University of Maryland School of Medicine, Baltimore 21201.
Proc Natl Acad Sci U S A. 1987 Nov;84(22):8169-73. doi: 10.1073/pnas.84.22.8169.
An IgG1 mouse monoclonal antibody produced in response to immunization with rat brain homogenate reacted with endothelial cells in the central and peripheral nervous system. Because antibody reactivity was associated with endothelia that have a selective permeability barrier, the antibody was called anti-endothelial-barrier antigen (anti-EBA). Paraffin sections of Bouins'-fixed rat tissue were used for initial screening and subsequent characterization of antibody reactivity. The antibody was generally unreactive with endothelial cells in other organs and with nonendothelial cells in or outside of the nervous system. Antibody binding was greatly reduced or absent in endothelia of the area postrema and choroid plexus, sites known to possess fenestrated blood vessels. In developing rat brain, anti-EBA binding to some microvessels was seen at 3 days postnatally. Anti-EBA reactivity outside the nervous system occurred in spleen and skin. Patchy reaction with portions of some spleen blood vessels and binding to some cells in the spleen were observed. In the skin, small cells, tentatively identified as Langerhans cells, which participate in Ia presentation, were stained. On immunoblots of rat brain microvessel preparations electrophoresed in Na-DodSO4/polyacrylamide gels, anti-EBA reacted with a protein triplet of Mr 30,000, 25,000, and 23,500 components.
一种因用大鼠脑匀浆免疫而产生的IgG1小鼠单克隆抗体，与中枢和外周神经系统中的内皮细胞发生反应。由于抗体反应性与具有选择性通透屏障的内皮细胞相关，该抗体被称为抗内皮屏障抗原（anti-EBA）。用Bouin氏固定的大鼠组织石蜡切片用于抗体反应性的初步筛选和后续鉴定。该抗体通常与其他器官中的内皮细胞以及神经系统内外的非内皮细胞无反应。在最后区和脉络丛的内皮细胞中，抗体结合大大减少或不存在，已知这些部位具有有孔血管。在新生大鼠脑中，出生后3天时可观察到anti-EBA与一些微血管结合。anti-EBA在神经系统外的反应出现在脾脏和皮肤中。观察到与部分脾血管有斑片状反应以及与脾中的一些细胞结合。在皮肤中，初步鉴定为参与Ia呈递的朗格汉斯细胞的小细胞被染色。在Na-DodSO4/聚丙烯酰胺凝胶中电泳的大鼠脑微血管制剂的免疫印迹上，anti-EBA与分子量为30,000、25,000和23,500的蛋白质三联体发生反应。
