Hassani Y, Saad J, Terrer E, Aboudharam G, Giancarlo B, Silvestri F, Raoult D, Drancourt M, Grine G
Aix-Marseille-Univ., IRD, MEPHI, IHU Méditerranée Infection, 19-21 Boulevard Jean Moulin, Marseille 13005, France.
IHU Méditerranée Infection, Marseille 13005, France.
Curr Res Microb Sci. 2021 Dec 20;3:100100. doi: 10.1016/j.crmicr.2021.100100. eCollection 2022.
Nanoarchaeota, obligate symbiont of some environmental archaea with reduced genomes, have been described in marine thermal vent environments, yet never detected in hosts, including humans.
Here, using laboratory tools geared towards the detection of nanoarchaea including PCR-sequencing, WGS, microscopy and culture.
We discovered a novel nanoarchaea, , detected in dental plate samples by specific PCR-based assays. Combining fluorescent hybridization (FISH) with scanning electron microscopy disclosed close contacts between and the archaea in these samples. Culturing one sample yielded co-isolation of and with a 606,935-bp genome, with 23.6% GC encoded 16 tRNA, 3 rRNA and 942 coding DNA sequences, of which 400 were assigned to clusters of orthologous groups.
The discovery of made publicly available in collection, extended our knowledge of human microbiota diversity, opening a new field of research in clinical microbiology here referred to as clinical nanoarchaeology.
纳古菌是一些基因组缩减的环境古菌的专性共生菌，已在海洋热液喷口环境中被描述，但从未在包括人类在内的宿主中被检测到。
在此，我们使用了针对纳古菌检测的实验室工具，包括聚合酶链反应测序、全基因组测序、显微镜检查和培养。
我们发现了一种新型纳古菌，通过基于聚合酶链反应的特异性检测方法在牙板样本中检测到。将荧光原位杂交（FISH）与扫描电子显微镜相结合，揭示了这些样本中该纳古菌与古菌之间的紧密接触。对一个样本进行培养，共分离出该纳古菌和另一种古菌，其基因组大小为606,935碱基对，GC含量为23.6%，编码16种转运RNA、3种核糖体RNA和942个编码DNA序列，其中400个被归类到直系同源簇。
该纳古菌的发现已在菌种保藏中心公开，扩展了我们对人类微生物群多样性的认识，开创了临床微生物学中一个新的研究领域，在此称为临床纳古菌学。
