Center for Soft Condensed Matter Physics and Interdisciplinary Research, School of Physical Science and Technology, Jiangsu Key Laboratory of Thin Films, Soochow University, Suzhou 215006, P. R. China.
J Mater Chem B. 2022 Mar 2;10(9):1434-1441. doi: 10.1039/d1tb02746h.
Surface-enhanced Raman scattering (SERS), which is based on the surface plasmon resonance (LSPR) of noble metal nanostructures, is widely used in the biological field due to its advantages of non-damaging samples and detection up to the molecular level. For biological SERS detection, preparation of substrates with biocompatibility and specific adsorption, leading to selective enhancement of the target biomolecules, are important design strategies. Utilizing the specific interaction between a carbohydrate and protein, a glycopolymer-based composite surface is fabricated to realize specific SERS detection of proteins. Herein, we use -3,4-dihydroxybenzeneethyl methacrylamide (DMA), 2-deoxy-2-(methacrylamido)glucopyranose (MAG) and methacrylic acid (MAA) as monomers in a sunlight-induced RAFT polymerization to synthesize a dopamine-containing glycopolymer. The glycopolymers are used to prepare a SERS substrate. The composite surface shows specific protein adsorption capacity, and the selective Raman enhancement of specific proteins was successfully achieved between the two different proteins Con A and BSA. This provides a feasible approach to design a SERS surface for protein detection and the study of the interaction between sugar and proteins.
表面增强拉曼散射（SERS）基于贵金属纳米结构的表面等离子体共振（LSPR），由于其对样品无损且可检测至分子水平的优点，在生物领域得到了广泛应用。对于生物 SERS 检测，制备具有生物相容性和特定吸附能力的基底，从而对目标生物分子进行选择性增强，是重要的设计策略。利用碳水化合物和蛋白质之间的特异性相互作用，制备了基于糖聚合物的复合表面，以实现对蛋白质的特异性 SERS 检测。在这里，我们使用 -3,4-二羟基苯乙基甲基丙烯酰胺（DMA）、2-脱氧-2-（甲基丙烯酰胺基）吡喃葡萄糖（MAG）和甲基丙烯酸（MAA）作为单体，在阳光诱导的 RAFT 聚合中合成了一种含有多巴胺的糖聚合物。将糖聚合物用于制备 SERS 基底。该复合表面表现出特定的蛋白质吸附能力，并成功实现了两种不同蛋白质 ConA 和 BSA 之间的特异性蛋白质的 Raman 选择性增强。这为设计用于蛋白质检测和糖与蛋白质相互作用研究的 SERS 表面提供了一种可行的方法。
