Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China.
College of Chemistry, University of Chinese Academy of Sciences, Beijing 100049, P. R. China.
ACS Appl Bio Mater. 2021 May 17;4(5):4549-4556. doi: 10.1021/acsabm.1c00283. Epub 2021 Apr 19.
3D bioprinting offers a powerful tool to fabricate vessel channels in tissue engineering applications, but inadequate strength of the vascular walls limited the development of this strategy and reinforced channels were highly desired for vascular constructions. Herein, we demonstrated a dual cross-linking system for 3D bioprinting of tubular structures, achieved by a combination of photo-cross-linking and enzymatic cross-linking. Photo-cross-linking of gelatin methacryloyl (GelMA) was achieved with a photoactive conjugated polymer PBF under 550 nm irradiation. Enzymatic cross-linking utilized cascade reactions catalyzed by glucose peroxidase and horseradish peroxidase that can cross-link both methacrylate and tyrosine moieties of GelMA. After removing the 3D-printed sacrificial layer (Pluronic F-127), the obtained perfusable channels showed great biocompatibility that allowed endothelial cells to adhere and proliferate. Our dual cross-linking strategy has great potential in 3D bioprinting of tubular structure for biomedical applications, especially for artificial blood vessels.
3D 生物打印为组织工程应用中制造血管通道提供了强大的工具，但血管壁的强度不足限制了该策略的发展，因此对于血管构建，人们强烈需要增强型通道。在此，我们展示了一种用于管状结构 3D 生物打印的双重交联系统，该系统通过光交联和酶交联的组合来实现。在 550nm 照射下，用光活性共轭聚合物 PBF 实现了明胶甲基丙烯酰（GelMA）的光交联。酶交联利用葡萄糖氧化酶和辣根过氧化物酶催化的级联反应来实现，该反应可以交联 GelMA 的甲基丙烯酰基和酪氨酸部分。去除 3D 打印的牺牲层（Pluronic F-127）后，得到的可灌注通道表现出良好的生物相容性，允许内皮细胞黏附和增殖。我们的双重交联策略在用于生物医学应用的管状结构 3D 生物打印方面具有巨大潜力，特别是对于人工血管。
