The State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, China.
J Cell Physiol. 2022 Aug;237(8):3408-3420. doi: 10.1002/jcp.30810. Epub 2022 Jun 14.
Little is known about the complex molecular and cellular events occurring during implantation, which represents a critical step for pregnancy. The conventional 2D culture could not support postimplantation embryos' normal development, and 3D conditions shed light into the "black box". 3D printing technology has been widely used in recapitulating the structure and function of native tissues in vitro. Here, we 3D printed anisotropic microporous scaffolds to culture embryos by manipulating the advancing angle between printed layers, which affected embryo development. The 30° and 60° scaffolds promote embryo development with moderate embryo-scaffold attachments. T-positive cells and FOXA2-positive cells were observed to appear in the posterior region of the embryo and migrated to the anterior region of the embryo on day 7. These findings demonstrate a 3D printed stand that supports embryonic development in vitro and the critical role of 3D architecture for embryo implantation, in which additive manufacturing is a versatile tool.
关于着床过程中发生的复杂分子和细胞事件知之甚少，着床是妊娠的关键步骤。传统的 2D 培养方式不能支持着床后胚胎的正常发育，而 3D 条件则揭示了“黑箱”。3D 打印技术已广泛应用于体外再现天然组织的结构和功能。在这里，我们通过操纵打印层之间的推进角度来 3D 打印各向异性微孔支架来培养胚胎，这影响了胚胎的发育。30°和 60°支架通过适度的胚胎-支架附着促进胚胎发育。在第 7 天，观察到 T 阳性细胞和 FOXA2 阳性细胞出现在胚胎的后区域，并迁移到胚胎的前区域。这些发现表明，3D 打印支架支持胚胎在体外的发育，3D 结构对于胚胎着床具有重要作用，其中增材制造是一种多功能工具。
