Department of Biosciences and Bioengineering, Indian Institute of Technology (IIT) Bombay, Powai, Mumbai 400076, India.
Centre for Research in Nano Technology & Science (CRNTS), Indian Institute of Technology (IIT) Bombay, Powai, Mumbai 400076, India.
ACS Appl Bio Mater. 2021 May 17;4(5):4641-4651. doi: 10.1021/acsabm.1c00379. Epub 2021 Apr 29.
Red emissive carbon dots from sucrose (SCD) were synthesized using a facile, isolation-free, one-pot method microwave pyrolysis. Various passivation agents were used along with sucrose, and a relative change in the chemical and optical properties of the carbon dots was investigated. A detailed systematic study of the effect of various passivations, different solvents, pHs, and temperatures on optical properties was carried out. The influence of excitation wavelength and passivation on photoluminescence (PL) is discussed considering the functional groups associated with the passivating agents. The effect of different solvents on dispersibility and PL behavior has been understood in terms of the dielectric properties of the solvents. The decrease in PL intensity of SCD from pH 3 to 11 facilitates pH sensing. The PL of SCD was found to be essentially stable between the temperature range of 20 and 80 °C. Additionally, the effects of physicochemical properties with respect to passivation, such as charge and surface chemistry in determining the cellular uptake and cytotoxicity, are also addressed. Aside from sensors, the potential of SCDs as bioimaging agents has also been studied for mammalian cells. Moreover, SCD exhibits excellent PL stability investigated under different storage conditions for 15 days.
采用简便、无分离、一锅微波热解法合成了来自蔗糖（SCD）的红色发射碳点。使用各种钝化剂与蔗糖一起，研究了碳点的化学和光学性质的相对变化。详细系统地研究了各种钝化剂、不同溶剂、pH 值和温度对光学性质的影响。考虑到与钝化剂相关的官能团，讨论了激发波长和钝化对光致发光（PL）的影响。根据溶剂的介电特性，理解了不同溶剂对分散性和 PL 行为的影响。SCD 的 PL 强度从 pH 3 到 11 的降低有助于 pH 感应。发现 SCD 的 PL 在 20 至 80°C 的温度范围内基本稳定。此外，还讨论了与钝化相关的物理化学性质（如电荷和表面化学）对细胞摄取和细胞毒性的影响。除了传感器，还研究了 SCD 作为生物成像剂在哺乳动物细胞中的潜在应用。此外，在 15 天的不同储存条件下研究了 SCD 的 PL 稳定性，结果表明其稳定性良好。
