Engineering Research Center for Nanomaterials, Henan University, Kaifeng 475004, China.
ACS Appl Bio Mater. 2021 Aug 16;4(8):6549-6557. doi: 10.1021/acsabm.1c00674. Epub 2021 Aug 2.
The fabrication of highly active and free-standing surface-enhanced Raman scattering (SERS) substrates in a simple and low-cost manner has been a crucial and urgent challenge in recent years. Herein, SiO nanofiber substrates modified with size-tunable Ag nanoparticles were prepared by the combination of electrospinning and in situ chemical reduction. The results demonstrate the presence and uniform adsorption of Ag nanoparticles on the SiO matrix surface. The free-standing composite nanofibrous substrates show high-performance SERS response toward 4-mercaptophenol and 4-mercaptobenzoic acid, and the detection limit can be as low as 10 mol/L. Most importantly, the as-prepared substrate as a versatile SERS platform can realize label-free detection of bio-macromolecules of bacteria, i.e., () and (). Furthermore, the substrates also possess outstanding antibacterial activities against and . Briefly, the significance of this study is that size-tunable Ag nanoparticles can be decorated on SiO nanofiber surfaces with triethanolamine as a bridging and reducing agent through a one-pot reaction, and the as-prepared nanofibrous membranes are expected to act as a candidate for label-free SERS detection as well as antibacterial dressing.
以简单且低成本的方式制备具有高活性和独立支撑的表面增强拉曼散射（SERS）基底，是近年来的一个关键且紧迫的挑战。在此，通过静电纺丝和原位化学还原相结合的方法，制备了具有尺寸可调 Ag 纳米颗粒的 SiO 纳米纤维基底。结果表明，Ag 纳米颗粒存在于 SiO 基体表面并均匀吸附。独立支撑的复合纳米纤维基底对 4-巯基苯酚和 4-巯基苯甲酸表现出高性能的 SERS 响应，检测限可低至 10 摩尔/升。最重要的是，所制备的基底作为一种通用的 SERS 平台，可以实现对细菌的生物大分子（）和（）的无标记检测。此外，该基底对 和 还具有出色的抗菌活性。简而言之，本研究的意义在于，通过一锅反应可以使用三乙醇胺作为桥连和还原剂，在 SiO 纳米纤维表面上修饰尺寸可调的 Ag 纳米颗粒，所制备的纳米纤维膜有望作为无标记 SERS 检测以及抗菌敷料的候选材料。
