College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, China.
College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, China.
J Colloid Interface Sci. 2022 Mar;609:718-733. doi: 10.1016/j.jcis.2021.11.075. Epub 2021 Nov 18.
Enhancing long-term antibacterial activity of membrane materials is an effective strategy to reduce biological contamination. Herein, we developed a long-term, synergistic antibacterial polyacrylonitrile (PAN) nanofiber membrane by a "one-pot" electrospinning process. In the reaction solution of PAN and N, N-dimethylformamide (DMF), silver-silicon dioxide nanoparticles (Ag@SiO NPs) are in-situ synthesized and stabilized using silane coupling agent; and [2-(methacryloyloxy)-ethyl] trimethylammonium chloride (MT) monomers are then in-situ cross-linked to obtain a polyquaternary ammonium salt (PMT). Subsequently, the casting solution is directly used to fabricate Ag@SiO/PMT-PAN nanofibrous membrane (NFM) via electrospinning. The antibacterial activity, reusability, synergy effect and biological safety of the Ag@SiO/PMT-PAN NFM are systematically investigated, and the synergistic antibacterial mechanism is also explored. Even at very low (0.3 wt%) content of silver, the Ag@SiO/PMT-PAN NFM exhibits excellent antibacterial activity against E. coli (99%) and S. aureus (99%). Also, the antibacterial ability of the NFM remains the same level after three cycles of antibacterial processes with the efficient synergy effects of Ag@SiO and PMT components. When the Ag@SiO/PMT-PAN contacts with bacteria, the PMT attracts and kills the bacteria through electrostatic action. The bacteria with damaged cell membranes are deposited on the nanofibrous membrane, which could greatly promote the release of Ag and further enhance the antibacterial activity. Moreover, L929 fibroblasts are co-cultured with the extract of 4 mg/mL Ag@SiO/PMT-PAN for 5 days, which exhibits a low cytotoxicity with a cell proliferation ratio of 95%. This work opens new pathways for developing long-term effective and synergistic antibacterial nanofibrous membrane materials to prevent infections associated with biomedical equipment.
提高膜材料的长期抗菌活性是减少生物污染的有效策略。本文通过“一锅法”静电纺丝工艺，制备了一种具有长期协同抗菌性能的聚丙烯腈（PAN）纳米纤维膜。在 PAN 和 N，N-二甲基甲酰胺（DMF）的反应溶液中，银-二氧化硅纳米颗粒（Ag@SiO NPs）原位合成并通过硅烷偶联剂稳定；然后将[2-（甲基丙烯酰氧基）乙基]三甲基氯化铵（MT）单体原位交联，得到聚季铵盐（PMT）。随后，将铸膜液直接用于静电纺丝制备Ag@SiO/PMT-PAN 纳米纤维膜（NFM）。系统研究了 Ag@SiO/PMT-PAN NFM 的抗菌活性、可重复使用性、协同效应和生物安全性，并探讨了协同抗菌机制。即使银的含量非常低（0.3wt%），Ag@SiO/PMT-PAN NFM 对大肠杆菌（99%）和金黄色葡萄球菌（99%）仍表现出优异的抗菌活性。此外，NFM 在经过三次抗菌处理循环后，仍保持相同的抗菌能力，Ag@SiO 和 PMT 成分具有高效的协同效应。当 Ag@SiO/PMT-PAN 与细菌接触时，PMT 通过静电作用吸引并杀死细菌。带有受损细胞膜的细菌沉积在纳米纤维膜上，这可以极大地促进 Ag 的释放，进一步增强抗菌活性。此外，将浓度为 4mg/mL 的 Ag@SiO/PMT-PAN 提取物与 L929 成纤维细胞共同培养 5 天，细胞增殖率为 95%，细胞毒性较低。这项工作为开发长期有效的协同抗菌纳米纤维膜材料以预防与生物医学设备相关的感染开辟了新途径。
