Yang Yan, Zhang Zhijie, Wan Menghui, Wang Zhihua, Zhao Yanbao, Sun Lei
Engineering Research Center for Nanomaterials, Henan University, Kaifeng 475004, China.
Ministry of Education Key Laboratory of Advanced Civil Engineering Material, School of Materials Science and Engineering, and Institute for Advanced Study, Tongji University, Shanghai 201804, China.
ACS Omega. 2020 Jul 30;5(31):19834-19843. doi: 10.1021/acsomega.0c02735. eCollection 2020 Aug 11.
Surface-enhanced Raman spectroscopy (SERS) can be applied for biological detection because of its high sensitivity and noninvasiveness for analytes. Herein, we engineer plasmonic free-standing substrates composed of Ag nanoparticles (NPs) supported on polyacrylonitrile (PAN) electrospinning nanofibrous felts as sensors for bacterial detection. Ag NPs are evenly distributed on PAN nanofibers after preimpregnation and impregnation of PAN nanofibers in Tollens' reagent. The size and loading density of Ag NPs are tunable by adjusting the reaction time of glucose and Tollens' reagent, thereby allowing the tuning of the surface plasmon resonance. Using 4-mercaptophenol (4-MPh) and 4-mercaptobenzoic acid (4-MBA) as probe molecules, SERS effects of Ag@PAN composite nanofibers are investigated, and the substrates allow the detection of 4-MPh and 4-MBA at a low concentration of 10 mol/L. Importantly, the substrates exhibit a high sensitivity of SERS performance for bacterial identification without a specific bacteria-aptamer conjugation. The SERS substrates also show good uniformity of SERS response for bacterial organelles. Furthermore, the antimicrobial property was evaluated, and the results indicate that the sample of Ag@PAN nanofiber mats possesses excellent antibacterial properties against and .
表面增强拉曼光谱（SERS）因其对分析物具有高灵敏度和非侵入性，可应用于生物检测。在此，我们设计了由负载在聚丙烯腈（PAN）静电纺丝纳米纤维毡上的银纳米颗粒（NPs）组成的等离子体独立式基底，作为细菌检测传感器。在将PAN纳米纤维预浸渍和浸渍在托伦试剂中后，银纳米颗粒均匀分布在PAN纳米纤维上。通过调节葡萄糖与托伦试剂的反应时间，可调节银纳米颗粒的尺寸和负载密度，从而实现表面等离子体共振的调谐。以4-巯基苯酚（4-MPh）和4-巯基苯甲酸（4-MBA）作为探针分子，研究了Ag@PAN复合纳米纤维的SERS效应，该基底能够检测低至10 mol/L浓度的4-MPh和4-MBA。重要的是，该基底在无需特异性细菌适配体结合的情况下，对细菌鉴定表现出高灵敏度的SERS性能。SERS基底对细菌细胞器的SERS响应也表现出良好的均匀性。此外，还评估了抗菌性能，结果表明Ag@PAN纳米纤维毡样品对 和 具有优异的抗菌性能。
