Institute of Nano Science and Technology, Sector 81, Manauli, SAS Nagar, Mohali, Punjab 140306, India.
ACS Appl Bio Mater. 2021 Aug 16;4(8):6203-6208. doi: 10.1021/acsabm.1c00524. Epub 2021 Jul 12.
In view to develop an autonomous lab-on-a-chip device for detection of toxins without using any spectroscopic or electrochemical equipment, self-powered enzyme micropumps were fabricated via layer-by-layer assembly of enzymes and polyelectrolytes. The thin film-based enzyme micropumps turned on fluid flow in the presence of respective substrates in a concentration-dependent manner, and the rate of the enzymatic reaction was the key for maneuvering the fluid flow. Furthermore, the newly engineered enzyme-based micropumps were able to detect toxic metals and organophosphorus pesticides by modulating the fluid flow speed as the rate of the enzymatic reaction was altered by the presence of inhibitors. Thus, by regulating fluid flow in a micropump, low concentrations of analytes (e.g., target biomarkers and inhibitors) in biological fluids can be quantitatively identified for testing in a resource-constrained environment.
为了开发无需使用任何光谱或电化学设备即可检测毒素的自主式微流控芯片设备，通过层层组装酶和聚电解质来制造自供电酶微泵。基于薄膜的酶微泵在存在各自底物的情况下以浓度依赖的方式开启流体流动，并且酶反应的速率是操纵流体流动的关键。此外，通过调节流体流动速度，新设计的基于酶的微泵可以检测有毒金属和有机磷农药，因为酶反应的速率会因抑制剂的存在而发生变化。因此，通过调节微泵中的流体流动，可以定量识别生物流体中低浓度的分析物（例如，靶标生物标志物和抑制剂），以便在资源有限的环境中进行测试。
