Organic and Polymer Synthesis Laboratory, Department of Chemistry, National Institute of Technology, Tiruchirappalli 620 015, India.
Department of Applied Chemistry, National Chiao Tung University, Hsinchu 30010, Taiwan, ROC.
ACS Appl Bio Mater. 2021 Sep 20;4(9):7007-7015. doi: 10.1021/acsabm.1c00647. Epub 2021 Sep 7.
Detection of chemical warfare agents (CWA) by simple and rapid methods with real-sample applications are quite inevitable in order to ease the threats to living systems caused by uncertain terror attacks and wars. Herein we have developed the first far-red to near infra-red (NIR) probe based on a covalent assembly approach for the detection of trace amounts of nerve agent mimic diethyl chloro phosphate (DCP) in soil and their fluorescent bio imaging in live cells. The probe features abrupt fluorescence turn on sensing of DCP with fluorescence quantum yield Φ = 0.622. It senses DCP selectively over other analytes in excellent sensitivity with a detection limit of 6.9 nM. In real time, the probe treated strips were employed to detect the DCP vapor effectively with eye catching fluorescence response. The presence of trace amounts of these acute warfare agents in the environment were monitored by soil analysis. Further fluorescent bio imaging was carried out to monitor trace level DCP in living cells using the HeLa cell line.
为了缓解不确定的恐怖袭击和战争对生命系统造成的威胁，用简单、快速的方法对化学战剂（CWA）进行检测，并实际应用于现场分析，这是非常必要的。在此，我们开发了首例基于共价组装策略的远红光近红外（NIR）探针，用于检测土壤中痕量神经毒剂类似物二乙基氯膦（DCP），并对活细胞中的荧光生物成像。该探针具有明显的 DCP 荧光猝灭传感特性，荧光量子产率Φ=0.622。它对 DCP 具有选择性和高灵敏度的响应，检测限低至 6.9 nM。在实时检测中，探针处理条带可用于有效检测 DCP 蒸气，具有醒目荧光响应。通过土壤分析监测环境中痕量的这些急性战剂的存在。进一步使用 HeLa 细胞系进行荧光生物成像，以监测活细胞中痕量 DCP 的水平。
