Regenerative Biomaterials Laboratory, Department of Bioengineering, Faculty of Engineering, Canakkale Onsekiz Mart University, Canakkale 17100, Turkey.
Personalized Medicine and Biosensing Research (PMBR) Laboratory, Chemistry Department, Faculty of Science, Ankara University, Ankara 06560, Turkey.
ACS Appl Bio Mater. 2021 Sep 20;4(9):7266-7279. doi: 10.1021/acsabm.1c00805. Epub 2021 Sep 5.
Tissue engineering and regenerative medicine have evolved into a different concept, the so-called clinical tissue engineering. Within this context, the synthesis of next-generation inorganic-organic hybrid constructs without the use of chemical crosslinkers emerges with a great potential for treating bone defects. Here, we propose a sophisticated approach for synthesizing cost-effective boron (B)- and silicon (Si)-incorporated collagen/hair keratin (B-Si-Col-HK) cryogels with the help of sol-gel reactions. In this approach, collagen and hair keratin were engaged with a B-Si network using tetraethyl orthosilicate as a silica precursor, and the obtained cryogels were characterized in depth with attenuated total reflectance-Fourier transform infrared spectroscopy, solid-state NMR, X-ray diffraction, thermogravimetric analysis, porosity and swelling tests, Brunauer-Emmett-Teller and Barrett-Joyner-Halenda analyses, frequency sweep and temperature-dependent rheology, contact angle analysis, micromechanical tests, and scanning electron microscopy with energy dispersive X-ray analysis. In addition, the cell survival and osteogenic features of the cryogels were evaluated by the MTS test, live/dead assay, immuno/histochemistry, and quantitative real-time polymerase chain reaction analyses. We conclude that the B-Si-networked Col-HK cryogels having good mechanical durability and osteoinductive features would have the potential bone formation capability.
组织工程和再生医学已经发展成为一个不同的概念，即所谓的临床组织工程。在这种背景下，合成下一代无机-有机杂化结构，而不使用化学交联剂，为治疗骨缺损带来了巨大的潜力。在这里，我们提出了一种复杂的方法，通过溶胶-凝胶反应来合成具有成本效益的硼（B）和硅（Si）掺入的胶原/角蛋白（B-Si-Col-HK）冷冻凝胶。在这种方法中，使用四乙氧基硅烷作为硅源，将胶原和角蛋白与 B-Si 网络结合，并用衰减全反射傅里叶变换红外光谱、固态 NMR、X 射线衍射、热重分析、孔隙率和溶胀试验、Brunauer-Emmett-Teller 和 Barrett-Joyner-Halenda 分析、频率扫描和温度依赖的流变学、接触角分析、微机械试验和带有能量色散 X 射线分析的扫描电子显微镜对所得冷冻凝胶进行了深入表征。此外，通过 MTS 试验、活/死检测、免疫/组织化学和定量实时聚合酶链反应分析评估了冷冻凝胶的细胞存活和成骨特性。我们得出结论，具有良好机械耐久性和成骨特性的 B-Si 网络 Col-HK 冷冻凝胶具有潜在的骨形成能力。
