Department of Genetics and Molecular Medicine, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
Department of Genetics and Molecular Medicine, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
J Clin Lab Anal. 2022 Feb;36(2):e24220. doi: 10.1002/jcla.24220. Epub 2022 Jan 10.
Prostate cancer (PCa) is a genetically heterogeneous disease with highly molecular aberrations. It has been revealed that a newly discovered class of non-coding RNAs called circular RNAs (circRNAs) play key roles in dictating tumor behaviors and phenotypes of the prostate tumors. In the current study, our aim was to determine the expression profiles of circHIAT1 and circCDR1AS in PCa compared with benign prostatic hyperplasia (BPH) tissues, as well as their clinicopathological relevance.
The 50 prostate tissues including 25 PCa tissues and 25 BPH samples were collected for analyzing the expression levels of target circRNAs by quantitative real-time PCR (qRT-PCR).
The results revealed that expression of circCDR1AS was significantly elevated in PCa compared with the BPH (p < 0.05). We also observed that PCa patients over the age of 60 had a higher expression of the circCDR1AS than patients under the age of 60 (p = 0.017). Moreover, a lower expression level of circHIAT1 was found in the PCa than BPH tissues (p < 0.05), and finally, the findings indicated that the area under the curve (AUC) of circCDR1AS was 0.848, with 92% sensitivity and 76% specificity, as well as an AUC of 0.828, with the 80% sensitivity and 76% specificity for circHIAT1.
These observations suggest that the abnormal expression of circCDR1AS and circHIAT1 can be regarded as two different types of molecular pathology with potential biomarker values for PCa, although further studies are needed.
前列腺癌（PCa）是一种遗传异质性疾病，具有高度的分子异常。现已发现一类新的非编码 RNA 称为环状 RNA（circRNAs），在决定前列腺肿瘤的肿瘤行为和表型方面发挥着关键作用。在本研究中，我们的目的是确定与良性前列腺增生（BPH）组织相比，circHIAT1 和 circCDR1AS 在 PCa 中的表达谱及其与临床病理的相关性。
收集了 50 例前列腺组织，包括 25 例 PCa 组织和 25 例 BPH 样本，通过实时定量 PCR（qRT-PCR）分析靶 circRNAs 的表达水平。
结果显示，circCDR1AS 在 PCa 中的表达明显高于 BPH（p<0.05）。我们还观察到，60 岁以上的 PCa 患者的 circCDR1AS 表达高于 60 岁以下的患者（p=0.017）。此外，circHIAT1 在 PCa 中的表达水平低于 BPH 组织（p<0.05），最终发现 circCDR1AS 的曲线下面积（AUC）为 0.848，灵敏度为 92%，特异性为 76%，circHIAT1 的 AUC 为 0.828，灵敏度为 80%，特异性为 76%。
这些观察结果表明，circCDR1AS 和 circHIAT1 的异常表达可以被视为两种不同类型的分子病理学，具有潜在的 PCa 生物标志物价值，但需要进一步研究。
