Heinemann D, Smith P J, Symes M O
Department of Surgery, University of Bristol, UK.
Br J Cancer. 1987 Oct;56(4):433-7. doi: 10.1038/bjc.1987.219.
Neoplastic tissue was obtained at operation from 10 renal cell carcinomas, from the adjacent 'normal' kidney in 6 cases and from 1 other normal kidney. The biopsies were snap frozen in liquid nitrogen and sections were subsequently stained with monoclonal antibodies against major histocompatibility complex (MHC) antigens, class I and II, and several types of mononuclear cell, by the indirect immunoperoxidase method. The degree of staining or the number of cells stained was estimated as heavy 4, through moderate 3, few 2, occasional 1, or nil 0. MHC Ag were consistently expressed, grade 2-4, by the glomeruli and proximal convoluted tubules of normal kidney, but were absent in 8 of 10 carcinomas. There was a grade 3-4 mononuclear cell infiltration in the stroma of normal kidney and between the carcinoma cells which was composed principally of macrophages. However in the two carcinomas expressing MHC Ag there was also a grade 2-3 infiltration with T lymphocytes. The absence of MHC Ag on carcinoma cells mitigates against attempts to potentiate the patient's immune response to his tumour, e.g. by renal artery embolisation.
手术时从10例肾细胞癌获取肿瘤组织，6例取自相邻的“正常”肾脏，另1例取自其他正常肾脏。活检组织在液氮中速冻，随后切片用抗主要组织相容性复合体（MHC）抗原I类和II类以及几种单核细胞的单克隆抗体，通过间接免疫过氧化物酶法染色。染色程度或染色细胞数量估计为重度4、中度3、少量2、偶尔1或无0。正常肾脏的肾小球和近端曲管始终表达MHC抗原，分级为2 - 4级，但10例癌中有8例无表达。正常肾脏间质和癌细胞之间有3 - 4级单核细胞浸润，主要由巨噬细胞组成。然而，在表达MHC抗原的2例癌中，也有2 - 3级T淋巴细胞浸润。癌细胞上MHC抗原的缺失不利于增强患者对肿瘤的免疫反应的尝试，例如通过肾动脉栓塞。
