Department of Neurology, Institute of Brain Science, Medical School, Shanxi Datong University, Datong, China.
Shanxi key Laboratory of Inflammatory Neurodegenerative Disease, Institute of Brain Science, Shanxi Datong University, Datong, China.
DNA Cell Biol. 2022 Feb;41(2):190-201. doi: 10.1089/dna.2021.0288. Epub 2022 Jan 10.
Hair follicle stem cells (HFSCs) play a significant role in hair development. miR-1 has been reported as an important regulatory factor that affects hair follicle growth and development, but its regulatory mechanism on HFSC development remains unknown. In this study, the molecular mechanism of miR-1 in regulating HFSC proliferation and differentiation was investigated. High-throughput RNA-seq and integrated analysis were performed to identify differentially transcribed mRNAs and microRNAs (miRNAs) in HFSCs co-cultured with dermal papilla cells (named dHFSCs) and control HFSCs. We then determined the molecular function of miR-1 in HFSCs. Compared with HFSCs, 13 differentially transcribed miRNAs were identified in dHFSCs. The results indicated that the overtranscription of miR-1 inhibited HFSC proliferation, but enhanced HFSC differentiation by targeting and genes. This study provides new insights into the molecular mechanisms of HFSC development. Approval ID (2014ZX08008-002).
毛囊干细胞（HFSCs）在毛发发育中起着重要作用。miR-1 已被报道为影响毛囊生长和发育的重要调节因子，但它对 HFSC 发育的调节机制尚不清楚。在这项研究中，研究了 miR-1 调节 HFSC 增殖和分化的分子机制。通过高通量 RNA-seq 和整合分析，鉴定了与真皮乳头细胞（命名为 dHFSCs）共培养的 HFSCs 和对照 HFSCs 中转录差异的 mRNAs 和 microRNAs（miRNAs）。然后，我们确定了 miR-1 在 HFSCs 中的分子功能。与 HFSCs 相比，在 dHFSCs 中鉴定出 13 个转录差异的 miRNAs。结果表明，miR-1 的过表达通过靶向 和 基因抑制 HFSC 增殖，但增强 HFSC 分化。这项研究为 HFSC 发育的分子机制提供了新的见解。审批号（2014ZX08008-002）。
