克隆和表达分析兰科植物 DnMSI1 基因
Cloning and expression analysis of DnMSI1 gene in orchid species Lindl.
机构信息
School of Biological Science and Agriculture, Qiannan Normal University, Duyun, Guizhou, China.
School of Environmental Science and Engineering, Guangzhou University, Guangzhou, Guangdong, China.
出版信息
Plant Signal Behav. 2022 Dec 31;17(1):2021649. doi: 10.1080/15592324.2021.2021649. Epub 2022 Jan 10.
WD40 repeat proteins, the homologs of yeast MSI1, are conserved in plants, participating in protein complexes and playing fundamental functions in plant development. Although several MSI1-like proteins have been cloned and characterized in plants, the roles of MSI1-like proteins in the biennial ornamental plant, Lindl, are still unclear. Here, we report the cloning of the gene from Lindl with RACE technology. We found that expression was induced by GA and TDZ but inhibited by ABA, PP333, and drought and salt stress. Furthermore, over-expression in resulted in decreased tolerance to NaCl stress. These results suggest that DnMSI1 plays negative regulation roles in regulating salinity-stress resistance in Lindl.
WD40 重复蛋白是酵母 MSI1 的同源物，在植物中保守，参与蛋白复合物，并在植物发育中发挥基本功能。尽管已经在植物中克隆和鉴定了几个 MSI1 样蛋白，但在两年生观赏植物 Lindl 中，MSI1 样蛋白的作用仍然不清楚。在这里，我们利用 RACE 技术从 Lindl 中克隆了 基因。我们发现 的表达受 GA 和 TDZ 的诱导，但受 ABA、PP333、干旱和盐胁迫的抑制。此外，在 中的过表达导致对 NaCl 胁迫的耐受性降低。这些结果表明，DnMSI1 在调控 Lindl 耐盐性中发挥负调控作用。
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