苹果（蔷薇科苹果属）基因的全基因组鉴定、表达及功能分析
Genome-wide Identification, Expression, and Functional Analysis of Genes in Apples ( Borkh.).
作者信息
Wang Daru, Wang Xun, Zhang Chunling, Yang Kuo, Wang Xinjie, Cui Jianying, Liu Dandan, You Chunxiang
机构信息
National Key Laboratory of Crop Biology, National Research Center for Apple Engineering and Technology, College of Horticulture Science and Engineering, Shandong Agricultural University, Taian, China.
College of Agriculture, Yunnan University, Kunming, China.
出版信息
Front Genet. 2022 Mar 3;13:846321. doi: 10.3389/fgene.2022.846321. eCollection 2022.
The multicopy suppressor of IRA (MSI) is a subfamily of WD40 repeat proteins, which is widely involved in plant growth and development. In order to explore the function of members in abiotic stress, we identified eight gene family members from the × reference genome. They were distributed on six chromosomes, and they had similar secondary and tertiary structures. We found a variety of regulatory elements in response to hormones and abiotic stress in promoters. Through qRT-PCR analysis, it was revealed that were expressed in all tissues, especially in roots. The analysis results also revealed that the expression of was induced in varying degrees under salt, drought stress, and ABA treatments. Furthermore, we obtained the overexpression of transgenic apple calli and . The overexpression of in calli and played a negative regulatory role in salt stress response. Our work laid a foundation for further verifying the function of genes under abiotic stress in apples.
IRA多拷贝抑制因子（MSI）是WD40重复蛋白的一个亚家族，广泛参与植物的生长发育。为了探究其成员在非生物胁迫中的功能，我们从×参考基因组中鉴定出8个基因家族成员。它们分布在6条染色体上，具有相似的二级和三级结构。我们在启动子中发现了多种响应激素和非生物胁迫的调控元件。通过qRT-PCR分析发现，它们在所有组织中均有表达，尤其是在根中。分析结果还表明，在盐、干旱胁迫和ABA处理下，其表达均受到不同程度的诱导。此外，我们获得了转基因苹果愈伤组织和的过表达植株。在愈伤组织中的过表达对盐胁迫响应起负调控作用。我们的工作为进一步验证苹果中基因在非生物胁迫下的功能奠定了基础。
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