Key Laboratory of Marine Genetics and Breeding of Ministry of Education of China, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.
College of Agronomy, Qingdao Agricultural University, Qingdao 266109, China.
Int J Mol Sci. 2021 Dec 23;23(1):145. doi: 10.3390/ijms23010145.
is a typical dinoflagellate that can cause harmful algal blooms, resulting in negative impacts on ecology and human health. The calcium (Ca) signal transduction pathway plays an important role in cell proliferation. Calmodulin (CaM) and CaM-related proteins are the main cellular Ca sensors, and can act as an intermediate in the Ca signal transduction pathway. In this study, the proteins that interacted with CaM of were screened by two-dimensional electrophoresis analysis and far western blots under different growth conditions including lag phase and high phosphorus and manganese induced log phase (HPM). The interactive proteins were then identified using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Four proteins were identified, including Ca/CaM-dependent protein kinase, serine/threonine kinase, annexin, and inositol-3-phosphate synthase, which all showed high expression levels under HPM. The gene expression levels encoding these four proteins were also up-regulated under HPM, as revealed by quantitative polymerase chain reaction, suggesting that the identified proteins participate in the Ca transport channel and cell cycle regulation to promote cell division. A network of proteins interacting with CaM and their target proteins involved in the regulation of cell proliferation was raised, which provided new insights into the mechanisms behind the explosive growth of .
是一种典型的甲藻，可引发有害藻华，对生态和人类健康造成负面影响。钙（Ca）信号转导途径在细胞增殖中起着重要作用。钙调蛋白（CaM）和 CaM 相关蛋白是主要的细胞 Ca 传感器，可作为 Ca 信号转导途径中的中间物。在这项研究中，通过二维电泳分析和远 Western 印迹法，在不同的生长条件下（包括迟滞期和高磷锰诱导的对数期（HPM））筛选与 相互作用的 CaM 蛋白。然后使用基质辅助激光解吸电离飞行时间质谱鉴定相互作用蛋白。鉴定到 4 种蛋白，包括 Ca/CaM 依赖性蛋白激酶、丝氨酸/苏氨酸激酶、膜联蛋白和肌醇-3-磷酸合酶，它们在 HPM 下均表现出高表达水平。定量聚合酶链反应显示这些 4 种蛋白的编码基因也在 HPM 下上调，表明鉴定到的蛋白参与 Ca 转运通道和细胞周期调控以促进细胞分裂。提出了一个与 CaM 相互作用的蛋白质及其参与细胞增殖调控的靶蛋白的网络，为 爆发性生长的机制提供了新的见解。
