Zhang Lidan, Yang Tingmi, Zhao Ying, Mo Zhitong, Yuan Zhixiang, Yang Yanchang, Wang Mingzhao, Liu Hongxia
Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), Nanjing Agricultural University, Nanjing, China.
Plant Dis. 2023 Feb;107(2):281-287. doi: 10.1094/PDIS-01-22-0157-RE. Epub 2023 Feb 7.
A bacterial pathogen strain was isolated from susceptible tissue of Hongyang variety kiwifruit in Zhongfeng Town, Ziyuan County, Guilin City, Guangxi, China. Due to the relatively single variety of kiwifruit in Guangxi, the control technology of fruit farmers is backward, and the climate is humid, which is suitable for the growth of pathogenic bacteria, resulting in frequent occurrence of diseases. In this study, the pathogen strain was identified based on morphological, physiological, and biochemical tests; 16S rRNA gene; PCR detection with specific primers; and Biolog analysis. The results showed that a tobacco allergic reaction could be induced by inoculation with the pathogenic bacteria. Additionally, brown necrotic plaques appeared on kiwifruit leaves, necrotic phloem lesions appeared, and wounds on kiwifruit branches turned brown. The characteristics identified by morphological, physiological, biochemical, and Biolog identification were similar to those caused by sp. Through 16S rRNA gene sequence analysis and PCR identification with specific primers, bands with a size corresponding to target bands indicated that the pathogen was subsp. . This is the first report of kiwifruit canker disease caused by subsp. in Guangxi, China. In addition, through this study, a preliminary understanding of the pathogen has been obtained, which will lay the foundation for the prevention and control of the disease in the future.
从中国广西桂林市资源县中峰镇红阳品种猕猴桃的感病组织中分离出一株细菌病原菌。由于广西猕猴桃品种相对单一，果农防治技术落后，且气候湿润，适宜病原菌生长，导致病害频发。本研究通过形态学、生理生化试验、16S rRNA基因、特异性引物PCR检测及Biolog分析对病原菌进行鉴定。结果表明，接种病原菌可诱发烟草过敏反应。此外，猕猴桃叶片上出现褐色坏死斑，韧皮部出现坏死病斑，猕猴桃枝条伤口变褐。通过形态学、生理生化及Biolog鉴定所确定的特征与 sp. 引起的特征相似。通过16S rRNA基因序列分析和特异性引物PCR鉴定，大小与目标条带对应的条带表明病原菌为 subsp. 。这是中国广西首次报道由 subsp. 引起的猕猴桃溃疡病。此外，通过本研究，对病原菌有了初步认识，为今后该病的防治奠定了基础。
