Institute for Surgical Pathology, Medical Center-University of Freiburg and Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany.
Clinic for Internal Medicine, Berlin Jewish Hospital, 13347 Berlin, Germany.
Int J Mol Sci. 2021 Dec 25;23(1):223. doi: 10.3390/ijms23010223.
We describe a sequential multistaining protocol for immunohistochemistry, immunofluorescence and CyTOF imaging for formalin-fixed, paraffin-embedded specimens (FFPE) in the formalin gas-phase (FOLGAS), enabling sequential multistaining, independent from the primary and secondary antibodies and retrieval. Histomorphologic details are preserved, and crossreactivity and loss of signal intensity are not detectable. Combined with a DAB-based hydrophobic masking of metal-labeled primary antibodies, FOLGAS allows the extended use of CyTOF imaging in FFPE sections.
我们描述了一种用于福尔马林固定、石蜡包埋（FFPE）标本的免疫组化、免疫荧光和 CyTOF 成像的顺序多重染色方案，该方案在福尔马林气相（FOLGAS）中进行，能够实现与一抗和二抗以及检索无关的顺序多重染色。保留了组织形态学细节，并且无法检测到交叉反应和信号强度的损失。与基于 DAB 的金属标记一抗的疏水性掩蔽相结合，FOLGAS 允许在 FFPE 切片中扩展使用 CyTOF 成像。
