使用福尔马林固定、石蜡包埋（FFPE）的小鼠肝组织切片进行免疫荧光鉴定枯否细胞和单核细胞衍生的巨噬细胞。

Kupffer Cell and Monocyte-Derived Macrophage Identification by Immunofluorescence on Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse Liver Sections.

机构信息

Department of Hepatology/Gastroenterology, Charité University Medical Center, Berlin, Germany.

Team Pathophysiology of Dyslipidemia, INSERM UMR1231 "Lipids, Nutrition, Cancer" and Université de Bourgogne Franche-Comté, Dijon, France.

出版信息

Methods Mol Biol. 2020;2164:45-53. doi: 10.1007/978-1-0716-0704-6_6.

DOI:10.1007/978-1-0716-0704-6_6

PMID:32607882

Abstract

Kupffer cells are the liver-resident macrophages and represent the first line of defense between the pathogens circulating from the intestines through the portal vein and systemic circulation. Recent works have highlighted the complex heterogeneity of macrophage functions and origins, thus raising awareness on the need for a better characterization of macrophage populations. The immunohistochemistry method here described, allows for a rapid distinction between Kupffer cells and monocyte-derived macrophages present on formalin-fixed, paraffin-embedded mouse liver samples. This protocol has been optimized for its reproducibility, reliability, and simplicity.

摘要

库普弗细胞是肝脏驻留的巨噬细胞，代表了从肠道通过门静脉和体循环循环的病原体与全身循环之间的第一道防线。最近的研究强调了巨噬细胞功能和起源的复杂异质性，从而提高了对更好地表征巨噬细胞群体的认识。本文描述的免疫组织化学方法可快速区分福尔马林固定、石蜡包埋的小鼠肝组织样本中的库普弗细胞和单核细胞衍生的巨噬细胞。该方案已针对其重现性、可靠性和简单性进行了优化。