针对曲霉菌种的特异性单克隆抗体的制备及其在曲霉菌病免疫组织化学鉴定中的应用。
Production of specific monoclonal antibodies to Aspergillus species and their use in immunohistochemical identification of aspergillosis.
作者信息
Fenelon L E, Hamilton A J, Figueroa J I, Bartholomew M A, Allen M H, McCarthy P, Hay R J
机构信息
Departments of Clinical Microbiology and Histopathology, St. Vincent's Hospital, Dublin 4, Ireland.
出版信息
J Clin Microbiol. 1999 Apr;37(4):1221-3. doi: 10.1128/JCM.37.4.1221-1223.1999.
Abstract
Two anti-Aspergillus murine monoclonal antibodies (MAbs), designated 164G and 611F, have been produced; both specifically recognize cytoplasmic antigens of A. fumigatus, A. flavus, and A. niger by enzyme-linked immunosorbent assay. The MAbs can identify Aspergillus spp. both in frozen sections by immunofluorescence and in paraffin-embedded clinical specimens by immunofluorescence and immunoperoxidase staining.
摘要
已制备出两种抗曲霉菌小鼠单克隆抗体（MAb），分别命名为164G和611F；通过酶联免疫吸附测定，二者均能特异性识别烟曲霉、黄曲霉和黑曲霉的细胞质抗原。这些单克隆抗体可通过免疫荧光法在冰冻切片中以及通过免疫荧光法和免疫过氧化物酶染色法在石蜡包埋的临床标本中鉴定曲霉菌属。
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