Department of Biomedical Engineering, Faculty of Fundamental Problems of Technology, Wrocław University of Science and Technology, 27 Wybrzeże S. Wyspiańskiego St., 50-370 Wroclaw, Poland.
Department of Pathology, Division of Microbiology, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, 31 C.K. Norwida St., 51-375 Wroclaw, Poland.
Int J Mol Sci. 2021 Dec 28;23(1):279. doi: 10.3390/ijms23010279.
The worldwide increase in bacterial resistance and healthcare-associated bacterial infections pose a serious threat to human health. The antimicrobial photodynamic method reveals the opportunity for a new therapeutic approach that is based on the limited delivery of photosensitizer from the material surface. Nanoporous inorganic-organic composites were obtained by entrapment of photosensitizer Photolon in polysiloxanes that was prepared by the sol-gel method. The material was characterized by its porosity, optical properties (fluorescence and absorbance), and laser-induced antimicrobial activity against , , , and . The permanent encapsulation of Photolon in the silica coating and the antimicrobial efficiency was confirmed by confocal microscope and digital holotomography. The generation of free radicals from nanoporous surfaces was proved by scanning Kelvin probe microscopy. For the first time, it was confirmed that Kelvin probe microscopy can be a label-free, noncontact alternative to other conventional methods based on fluorescence or chemiluminescence probes, etc. It was confirmed that the proposed photoactive coating enables the antibacterial photodynamic effect based on free radicals released from the surface of the coating. The highest bactericidal efficiency of the proposed coating was 87.16%. This coating can selectively limit the multiplication of bacterial cells, while protecting the environment and reducing the risk of surface contamination.
全球范围内细菌耐药性的增加和与医疗保健相关的细菌感染对人类健康构成了严重威胁。抗菌光动力方法为一种新的治疗方法提供了机会，该方法基于从材料表面有限地递送光敏剂。通过将光敏剂 Photolon 包埋在溶胶-凝胶法制备的聚硅氧烷中，获得了多孔无机-有机复合材料。该材料的多孔性、光学性质（荧光和吸收）以及对 、 、 、 和 的激光诱导抗菌活性进行了表征。通过共聚焦显微镜和数字全息术证实了 Photolon 在二氧化硅涂层中的永久包封和抗菌效率。通过扫描 Kelvin 探针显微镜证明了从纳米多孔表面产生自由基。首次证实 Kelvin 探针显微镜可以作为基于荧光或化学发光探针等其他传统方法的无标记、非接触替代方法。证实所提出的光活性涂层能够基于从涂层表面释放的自由基实现抗菌光动力效应。所提出的涂层具有最高 87.16%的杀菌效率。这种涂层可以选择性地限制细菌细胞的繁殖，同时保护环境并降低表面污染的风险。
