Chao C C, Lin-Chao S
Department of Biochemistry, University of Texas Health Science Center, Dallas 75235.
FEBS Lett. 1987 Dec 10;225(1-2):133-8. doi: 10.1016/0014-5793(87)81145-6.
The induction of enzymatic photorepair (EPR) in ICR 2A frog cells and a derived mutant cell line DRP36 hypersensitive to solar UV was studied. Using clonogenic assays, when induced wild-type cells demonstrated an 8-fold increase of EPR the mutant cells displayed a near-background level of inducible EPR. The constitutive EPR in mutant cells, however, was the same as in wild-type cells. A mixed culture of ICR 2A and DRP36 cells showed an intermediate inducible EPR depending upon the cell ratio. Inducible EPR was also detected at the DNA level in wild-type cells, but not in mutant cells.
研究了ICR 2A蛙细胞及对太阳紫外线超敏的衍生突变细胞系DRP36中酶促光修复（EPR）的诱导情况。通过克隆形成试验，当诱导野生型细胞的EPR增加8倍时，突变细胞的可诱导EPR显示出接近背景水平。然而，突变细胞中的组成型EPR与野生型细胞相同。ICR 2A和DRP36细胞的混合培养物显示出取决于细胞比例的中间可诱导EPR。在野生型细胞的DNA水平也检测到了可诱导EPR，但在突变细胞中未检测到。
