Chao C C, Lin-Chao S
Department of Biochemistry, University of Texas Health Science Center, Dallas 75235.
Mutat Res. 1987 Nov;192(3):211-6. doi: 10.1016/0165-7992(87)90058-3.
The constitutive and inducible levels of enzymatic photorepair (EPR) in growing and arrested ICR 2A frog cells were studied using clonogenic assays. Both arrested and growing cells exhibited an equal level of constitutive EPR following ultraviolet irradiation. However, only arrested, but not growing, cells treated with a low fluence of UV (90% survival) developed an enhanced EPR. The induced process developed transiently with a peak 3 days after pre-irradiation, and was totally blocked by a nontoxic concentration of cycloheximide. In addition, the induced EPR is unique to low fluences of UV.
利用克隆形成试验研究了生长和停滞状态的ICR 2A蛙细胞中酶促光修复（EPR）的组成型水平和诱导水平。在紫外线照射后，停滞细胞和生长细胞均表现出相同水平的组成型EPR。然而，仅用低剂量紫外线（90%存活率）处理的停滞细胞而非生长细胞出现了增强的EPR。诱导过程短暂发生，在预照射后3天达到峰值，并被无毒浓度的环己酰亚胺完全阻断。此外，诱导的EPR是低剂量紫外线所特有的。
