Rosenstein B S, Chao C C
Somat Cell Mol Genet. 1985 Jul;11(4):339-44. doi: 10.1007/BF01534410.
A mutant cell line DRP 36, hypersensitive to nondimer DNA damage induced by exposure of cells to the Mylar-filtered solar ultraviolet (UV) radiation produced by a fluorescent sunlamp plus photoreactivating light (PRL) was isolated from the haploid ICR 2A frog cell line. The DO for mutant cells exposed to this solar UV source was 3.3 kJ/m2 compared with a DO of 7.3 kJ/m2 for the parental ICR 2A cells. In contrast, DRP 36 and ICR 2A cells exhibited similar levels of survival following 254-nm irradiation which causes the induction primarily of pyrimidine dimers. The cross-sensitivity to additional DNA damaging agents was examined, and it was determined that the DRP 36 cells are also hypersensitive to treatment with gamma-rays, ethyl methane sulfonate (EMS), cis-dichlorodiammine platinum (II) (DDP), and 4-nitroquinoline oxide (4-NQO) while exhibiting normal sensitivity to L-phenylalanine mustard (L-PAM), 1,3-bis-(2-chloroethyl)-1-nitrosourea (BCNU) and mitomycin C (MMC).
从单倍体ICR 2A青蛙细胞系中分离出一种突变细胞系DRP 36，该细胞系对细胞暴露于荧光太阳灯产生的聚酯薄膜过滤的太阳紫外线（UV）辐射加光复活光（PRL）诱导的非二聚体DNA损伤高度敏感。暴露于这种太阳紫外线源的突变细胞的剂量为3.3 kJ/m²，而亲本ICR 2A细胞的剂量为7.3 kJ/m²。相比之下，DRP 36和ICR 2A细胞在254纳米辐射后表现出相似的存活率，254纳米辐射主要导致嘧啶二聚体的诱导。研究了对其他DNA损伤剂的交叉敏感性，确定DRP 36细胞对γ射线、甲基磺酸乙酯（EMS）、顺式二氯二氨铂（II）（DDP）和4-硝基喹啉氧化物（4-NQO）处理也高度敏感，而对L-苯丙氨酸氮芥（L-PAM）、1,3-双（2-氯乙基）-1-亚硝基脲（BCNU）和丝裂霉素C（MMC）表现出正常敏感性。
