Lee Siyoung, Park Girim, Choi Yunseo, Park Seoyeon, Kim Hoytaek, Lee Oakjin, Kim Taebok, Park Younghoon
Department of Horticultural Bioscience, Pusan National University, Miryang 50463, Korea.
Department of Horticulture, Sunchon National University, Sunchon 57922, Korea.
Plants (Basel). 2021 Dec 21;11(1):8. doi: 10.3390/plants11010008.
-lycopene is a functional phytochemical abundant in red-fleshed watermelons, and its contents vary among cultivars. In this study, the genetic basis of high -lycopene contents in scarlet red flesh was evaluated. Three near-isogenic lines (NILs) with high -lycopene contents were derived from the scarlet red-fleshed donor parent DRD and three coral red-fleshed (low -lycopene contents) recurrent parents. The lycopene contents of DRD (589.4 ± 71.8 µg/g) were two times higher than that of the recurrent parents, and values for NILs were intermediate between those of the parents. Coral red-fleshed lines and F1 cultivars showed low -lycopene contents (135.7 ± 18.0 µg/g to 213.7 ± 39.5 µg/g). Whole-genome resequencing of two NILs and their parents and an analysis of genome-wide single-nucleotide polymorphisms revealed three common introgressed regions (CIRs) on chromosomes 6, 9, and 10. Twenty-eight gene-based cleaved amplified polymorphic sequence (CAPS) markers were developed from the CIRs. The CAPS markers derived from CIR6 on chromosome 6, spanning approximately 1 Mb, were associated ( = 0.45-0.72) with the -lycopene contents, particularly CIR6-M1 and CIR6-M4. Our results imply that CIR6 is a major genomic region associated with variation in the -lycopene contents in red-fleshed watermelon, and CIR6-M1 and CIR6-M4 may be useful for marker-assisted selection.
番茄红素是一种在红肉西瓜中含量丰富的功能性植物化学物质，其含量因品种而异。在本研究中，对深红色果肉中高番茄红素含量的遗传基础进行了评估。三个高番茄红素含量的近等基因系（NILs）源自深红色果肉的供体亲本DRD和三个珊瑚红色果肉（低番茄红素含量）的轮回亲本。DRD的番茄红素含量（589.4±71.8μg/g）比轮回亲本高出两倍，NILs的值介于亲本之间。珊瑚红色果肉系和F1品种的番茄红素含量较低（135.7±18.0μg/g至213.7±39.5μg/g）。对两个NILs及其亲本进行全基因组重测序，并分析全基因组单核苷酸多态性，发现染色体6、9和10上有三个共同的渗入区域（CIRs）。从这些CIRs开发了28个基于基因的酶切扩增多态性序列（CAPS）标记。来自染色体6上CIR6的CAPS标记，跨度约1 Mb，与番茄红素含量相关（r = 0.45 - 0.72），特别是CIR6-M1和CIR6-M4。我们的结果表明，CIR6是与红肉西瓜中番茄红素含量变异相关的主要基因组区域，CIR6-M1和CIR6-M4可能有助于标记辅助选择。
