Xu Lu, Gao Fan, Feng Jia, Lv Junping, Liu Qi, Nan Fangru, Liu Xudong, Xie Shulian
School of Life Science, Shanxi University, Taiyuan 030006, China.
Plants (Basel). 2021 Dec 22;11(1):27. doi: 10.3390/plants11010027.
To study the relationship between β-carotene synthesis and geranylgeranyl pyrophosphate synthase (GGPS) activity, 15 species of were used to determine the changes in photosynthetic pigment contents, chlorophyll fluorescence parameters, β-carotene content, and GGPS activity. By observing the morphology and size of 15 species of , D8 has the largest individual algal cell and D9 has the smallest individual. Growth was relatively slow during days one through seven. After about eight days, the cells entered the logarithmic growth period and grew rapidly to a high density. After about 45 days, they entered a mature period, and growth slowed down. The contents of chlorophyll, carotenoids, and β-carotene increased during growth. D1 has the highest accumulation of β-carotene, and GGPS enzyme activity has a positive linear relationship with the β-carotene synthesis content. Phylogenetic analysis showed that the GGPS proteins of the 15 species were highly homologous, and the GGPS protein was not part of the membrane.
为研究β-胡萝卜素合成与香叶基香叶基焦磷酸合酶（GGPS）活性之间的关系，使用15种[藻类名称未给出]来测定光合色素含量、叶绿素荧光参数、β-胡萝卜素含量和GGPS活性的变化。通过观察15种[藻类名称未给出]的形态和大小，D8的单个藻细胞最大，D9的单个藻细胞最小。在第1天至第7天期间生长相对缓慢。大约8天后，细胞进入对数生长期并迅速生长至高密度。大约45天后，它们进入成熟期，生长减缓。生长过程中叶绿素、类胡萝卜素和β-胡萝卜素的含量增加。D1的β-胡萝卜素积累量最高，且GGPS酶活性与β-胡萝卜素合成含量呈正线性关系。系统发育分析表明，15种[藻类名称未给出]的GGPS蛋白高度同源，且GGPS蛋白不是膜的一部分。
