Sun Yinnan, Yang Kui, Cao Qin, Sun Jinde, Xia Yu, Wang Yinhang, Li Wei, Ma Chunhui, Liu Shouxin
College of Material Science and Engineering, Northeast Forestry University, Harbin 150040, China.
Molecules. 2017 Jul 11;22(7):1156. doi: 10.3390/molecules22071156.
A homogenate-assisted vacuum-powered bubble extraction (HVBE) method using ethanol was applied for extraction of flavonoids from leaves. The mechanisms of homogenate-assisted extraction and vacuum-powered bubble generation were discussed in detail. Furthermore, a method for the rapid determination of flavonoids by HPLC was established. HVBE followed by HPLC was successfully applied for the extraction and quantification of four flavonoids in , including orientin, isoorientin, vitexin, and isovitexin. This method provides a fast and effective means for the preparation and determination of plant active components. Moreover, the on-line antioxidant capacity, including scavenging positive ion and negative ion free radical capacity of different fractions from the bamboo flavonoid extract was evaluated. Results showed that the scavenging DPPH free radical capacity of vitexin and isovitexin was larger than that of isoorientin and orientin. On the contrary, the scavenging ABTS⁺free radical capacity of isoorientin and orientin was larger than that of vitexin and isovitexin.
采用一种使用乙醇的匀浆辅助真空动力气泡萃取（HVBE）方法从叶片中提取黄酮类化合物。详细讨论了匀浆辅助萃取和真空动力气泡产生的机制。此外，建立了一种通过高效液相色谱法快速测定黄酮类化合物的方法。HVBE 结合高效液相色谱法成功应用于竹子中四种黄酮类化合物（包括荭草苷、异荭草苷、牡荆素和异牡荆素）的提取和定量分析。该方法为植物活性成分的制备和测定提供了一种快速有效的手段。此外，还评估了竹子黄酮提取物不同馏分的在线抗氧化能力，包括清除正离子和负离子自由基的能力。结果表明，牡荆素和异牡荆素清除 DPPH 自由基的能力大于异荭草苷和荭草苷。相反，异荭草苷和荭草苷清除 ABTS⁺自由基的能力大于牡荆素和异牡荆素。
