Zamljen Tilen, Medič Aljaž, Veberič Robert, Hudina Metka, Jakopič Jerneja, Slatnar Ana
Department of Agronomy, Biotechnical Faculty, University of Ljubljana, SI-1000 Ljubljana, Slovenia.
Plants (Basel). 2021 Dec 29;11(1):101. doi: 10.3390/plants11010101.
Chilies are widely cultivated for their rich metabolic content, especially capsaicinoids. In our study, we determined individual sugars, organic acids, capsaicinoids, and total phenolic content in pericarp, placenta, and seeds of L., Jacq. and L. by HPLC/MS. Dry weight varied in the cultivar 'Cayenne', with the first fruit having the lowest dry weight, with 4.14 g. The total sugar content and organic acid content did not vary among the fruits of all three cultivars. The cultivar 'Cayenne' showed differences in total phenolic and capsaicinoid content between fruits in the placenta, with the first fruit having the highest content of total phenolics (27.85 g GAE/kg DW) and total capsaicinoids (16.15 g/kg DW). Of the three cultivars studied, the cultivar 'Habanero Orange' showed the least variability among fruits in terms of metabolites. The content of dihydrocapsaicin, nordihydrocapsaicin, homocapsaicin, and homodihydrocapsaicin in the seeds of the second fruit was higher than that of the first fruit of the cultivar 'Bishop Crown'. The results of our study provided significant insight into the metabolomics of individual fruits of the same chili plant. We have thus increased our understanding of how certain metabolites are distributed between fruits at different levels of the same plant and different parts of the fruit. This could be further investigated when chilies are exposed to different environmental stresses.
辣椒因其丰富的代谢物含量，尤其是辣椒素类物质而被广泛种植。在我们的研究中，我们通过高效液相色谱/质谱法测定了辣椒（L.）、辣椒（Jacq.）和辣椒（L.）的果皮、胎座和种子中的单糖、有机酸、辣椒素类物质和总酚含量。‘卡宴’品种的干重有所不同，第一个果实的干重最低，为4.14克。所有三个品种的果实中总糖含量和有机酸含量没有差异。‘卡宴’品种在胎座果实的总酚和辣椒素类物质含量上存在差异，第一个果实的总酚含量最高（27.85克没食子酸当量/千克干重），总辣椒素含量最高（16.15克/千克干重）。在所研究的三个品种中，‘哈瓦那橙’品种在果实代谢物方面的变异性最小。第二个果实种子中的二氢辣椒素、去甲二氢辣椒素、高辣椒素和高二氢辣椒素含量高于‘毕晓普皇冠’品种的第一个果实。我们的研究结果为同一辣椒植株单个果实的代谢组学提供了重要见解。我们因此加深了对某些代谢物如何在同一植株不同水平的果实以及果实不同部位之间分布的理解。当辣椒受到不同环境胁迫时，这一点可以进一步研究。
