Fatima Sidra, Zafar Zeeshan, Gul Alvina, Bhatti Muhammad Faraz
Atta-Ur-Rahman School of Applied Bioscience, National University of Sciences and Technology, H-12, Kashmir Highway, Islamabad 44000, Pakistan.
Plants (Basel). 2021 Dec 31;11(1):117. doi: 10.3390/plants11010117.
Stress-associated proteins (SAPs) are zinc finger proteins involved in the regulation of various stresses in a variety of plant species. A total of nine genes were identified in . Phylogenetic and synteny analyses were performed to analyze the homology and evolutionary relationship of genes. The functions of genes were assessed by further analyses, including -regulatory elements, gene duplication, gene ontology, gene structure, subcellular localization, and motif pattern. This study found that genes were unevenly distributed on chromosomes 2, 3, 6, and 7. Phylogenetic analysis of genes with and suggested that six subgroups have a similar pattern of AN1 and A20 domains in each subgroup. genes lacked duplicated blocks. The majority of genes were localized in the nucleus region. Three hormonal and five stress -regulatory elements were found in the upstream promoter region of the gene family. RNA-seq analysis revealed differential gene expression of genes at days 12, 17, 22, 27, 32, and 37 of fruitlet development after flowering. This study identifies the SAP genes in and also provides insights into the expression of genes in abnormal fruitlets with diapause atrophic growth at various developmental stages.
胁迫相关蛋白（SAPs）是参与多种植物物种中各种胁迫调控的锌指蛋白。在……中总共鉴定出9个基因。进行了系统发育和共线性分析以分析这些基因的同源性和进化关系。通过进一步分析评估这些基因的功能，包括顺式调控元件、基因复制、基因本体论、基因结构、亚细胞定位和基序模式。本研究发现这些基因在2号、3号、6号和7号染色体上分布不均。对这些基因与……的系统发育分析表明，六个亚组在每个亚组中具有相似的AN1和A20结构域模式。这些基因缺乏重复块。大多数这些基因定位于细胞核区域。在该基因家族的上游启动子区域发现了三个激素和五个胁迫顺式调控元件。RNA测序分析揭示了开花后果实发育第12、17、22、27、32和37天这些基因的差异基因表达。本研究鉴定了……中的SAP基因，还为这些基因在不同发育阶段具有滞育萎缩生长的异常小果实中的表达提供了见解。
